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Microbiological counts during beet sugar extraction.

S Robles-Gancedo1, T M López-Díaz, A Otero

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Summary

Microbial contamination, including gum-forming bacteria and yeasts, is significant during beet sugar extraction. Identifying these contamination points is key for implementing control measures in sugar processing.

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Area of Science:

  • Food Microbiology
  • Industrial Microbiology
  • Biotechnology

Background:

  • Microbial contamination poses significant challenges in food processing industries, impacting product quality and yield.
  • Beet sugar extraction is a critical industrial process susceptible to microbial spoilage.
  • Understanding microbial dynamics is essential for optimizing sugar production and preventing economic losses.

Purpose of the Study:

  • To identify dominant microbial populations during beet sugar extraction.
  • To pinpoint critical control points for microbial contamination within the extraction process.
  • To assess the impact of microbial contamination on sugar yield and quality.

Main Methods:

  • Investigated four Spanish beet sugar factories, sampling 18 stages per factory.
  • Analyzed microbial groups (mesophiles, thermophiles, yeasts, lactic acid bacteria, gum-forming bacteria) and physicochemical parameters.
  • Employed statistical analysis to determine significant differences in contamination levels.

Main Results:

  • Beets, cossettes, and diffusion juices exhibited the highest microbial contamination, primarily by mesophiles, thermophiles, and yeasts.
  • Significant differences in microbial contamination were observed among factories and process stages (P < 0.01).
  • Gum-forming bacteria and yeasts were abundant (>6 log CFU/g) in beets and cossettes; diffusion juices were heavily contaminated with aerobic mesophiles, lactic acid bacteria, and thermophiles.

Conclusions:

  • Beet washing water, equipment, and air are significant contamination sources.
  • Gum-forming bacteria persist through diffusion, potentially causing filter obstruction.
  • Microbial control strategies are needed to mitigate sugar loss and processing issues in beet sugar factories.