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Genotyping of strawberry (Fragaria x ananassa Duch.) cultivars by DNA markers: interlaboratory study
Miyuki Kunihisa1, Hiroshi Ueda, Nobuko Fukino
1National Institute of Vegetable and Tea Science, Kannondai 3-1-1, Tsukuba, Ibaraki 305-8666, Japan. miyuky@affrc.go.jp
Abstract:
Fourteen Japanese laboratories validated the reproducibility of genotyping by 25 cleavage amplified polymorphic sequence (CAPS) markers for discrimination of strawberry (Fragaria x ananassa Duch.) cultivars. Both the sensitivity and specificity rate of 12 markers were 100%, those of another 12 were >95%, and those of 1 were >90%. These results indicate that the method of genotyping by the CAPS markers was highly reproducible and could provide a useful basis for practical identification of strawberry cultivars. This is the first report of the statistical validation of crop genotyping by DNA markers.
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