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[Expression study of DNA translesion synthesis genes in human primary glioma]
Cai-hua Xi1, Jin Hu, Hui-bo Wang
1Shanghai Acute Trauma Care Centre, the Sixth People's Hospital of Shanghai Jiaotong University, Shanghai 200233, China.
Objective:
To detect the expressions of Rev1, Pol zeta, Pol eta, Pol iota and Pol kappa mRNA in human primary glioma tissues, and to analyze the relationship between the expression and the pathological grade.
Methods:
We applied SYBR Green real-time PCR to investigate the mRNA expressions of the 6 genes in 85 primary glioma tissues and 14 normal brain tissues.
Results:
The expressions of 4 genes including Rev3, Rev7, Pol eta and Pol iota were significantly (P < 0.05) higher in glioma tissues in grade II, III and IV as compared to normal brain tissues. The expressions of Rev1 and Pol kappa were significantly (P < 0.05) higher in grade IV glioma compared to the normal brain tissue. The positive correlation existed (P < 0.01) between the expression of all genes mRNA and the pathologic grade of the human glioma, except for Rev7.
Conclusion:
The up-regulation of DNA translesion synthesis genes, including Rev1, Rev3, Rev7, Pol eta, Pol iota and Pol kappa, may be associated with pathogenesis of glioma.
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