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Updated: Jun 21, 2026
Two-vessel Occlusion Mouse Model of Cerebral Ischemia-reperfusion
Published on: March 1, 2019
[Interfering effect of neuregulin upon cerebral ischemic reperfusion injury in rhesus monkeys]
1Institute of Cerebrovascular Diseases, Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, China.
Objective:
To study the interfering effect of neuregulin-1beta (NRG-1beta) on the cerebral ischemic reperfusion injury in monkeys.
Methods:
The models with middle cerebral artery occlusion reperfusion (MCAO/R) were established by inserting a micro-balloon catheter intra-arterially into MCA from femoral artery in 12 adult healthy monkeys. The NRG-1beta was injected into MCA from micro-balloon catheter in the animals of treatment group at 2 h post-ischemia while the normal saline was simultaneously injected in the animals of control group. Then the micro-balloon catheter was withdrawn from the MCA to perfuse for 22 h. The nervous behavioral function was evaluated by task-oriented score and the infarct volume measured by TTC and MRI.
Results:
The animals of control group had an onset of nervous functional disorders at 2 h post-ischemia and had no significant difference at reperfusion 22 h (59.8 +/- 15.7); the nervous function of monkeys had no improvement after NFG-1beta treatment (61.3 +/- 16.2) (P > 0.05). The diffuse weight imaging (DWI) showed a high signal area but T(1) and T(2) imaging had no significant changes at 2 h post-ischemia in control group animals; while the T(1) and T(2) imaging showed a typical high signal areas, DWI high signal area expanded and TTC staining ischemic area appeared at reperfusion 22 h. The T(1), T(2) and DWI abnormal signal areas and TTC cerebral infarct volume did not shrink significantly in NFG-1beta treatment group (P > 0.05).
Conclusion:
The neuroprotective effects of NRG-1beta upon cerebral ischemic reperfusion injury needs to be further studied.
Insights
Neuregulin-1beta (NRG-1beta) did not show significant neuroprotective effects in a monkey model of cerebral ischemic reperfusion injury. Further studies are needed to explore its potential therapeutic role in stroke. Keywords: NRG-1beta, stroke, neuroprotection, cerebral ischemia.
Area of Science:
- Neuroscience
- Cerebrovascular Research
- Biomedical Science
Context:
- Cerebral ischemic reperfusion injury is a major cause of stroke-related disability.
- Neuregulin-1beta (NRG-1beta) is a growth factor with potential roles in neuronal survival and repair.
- Investigating novel therapeutic targets for stroke is crucial.
Purpose:
- To evaluate the neuroprotective efficacy of Neuregulin-1beta (NRG-1beta) in a primate model of middle cerebral artery occlusion/reperfusion (MCAO/R).
- To assess the impact of NRG-1beta on neurological function and infarct volume following ischemic stroke.
Summary:
- Middle cerebral artery occlusion/reperfusion (MCAO/R) models were established in 12 monkeys.
- NRG-1beta or saline was administered intra-arterially 2 hours post-ischemia, followed by 22 hours of reperfusion.
- Neurological function (task-oriented score) and infarct volume (TTC, MRI) were measured. NRG-1beta treatment did not significantly alter neurological scores or infarct size compared to controls.
Impact:
- This study suggests that NRG-1beta may not offer significant neuroprotection against acute ischemic stroke in this primate model.
- The findings indicate that the neuroprotective effects of NRG-1beta in cerebral ischemic reperfusion injury require further investigation.
- Results highlight the complexity of therapeutic interventions for stroke and the need for continued research.
