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[Expression of angiogenic factors in pancreatic carcinoma cell and their significance]
1Department of Hepatobiliary Surgery, Second Hospital of Hebei Medical University, Shijiazhuang 050011, China.
Objective:
To investigate the relationship between expression of angiogenic factors and invasion and proliferation in pancreatic carcinoma cell.
Methods:
Expressions and secretions of angiogenic factors in pancreatic carcinoma cell lines were determined by RT-PCR and ELISA. Proliferation and invasion of pancreatic carcinoma cell lines were determined by CCK-8 and Boyden Chamber invasion tests.
Results:
Pancreatic carcinoma cell lines showed significantly different ability of invasion and proliferation. Intracellular VEGF expressions of SW1990, Capan-1, Aspc-1, MiaPaCa-2, Panc-1 and PCT-3 were (152.9 +/- 0.6), (224.1 +/- 60.3), (239.2 +/- 2.1), (19.3 +/- 0.7), (165.6 +/- 34.3), and (18.1 +/- 1.4) pg/(10(6)cell.24 h). Extracellular VEGF expressions of SW1990, Capan-1, Aspc-1, MiaPaCa-2, Panc-1 and PCT-3 were (1331.1 +/- 67.8), (3902.6 +/- 79.7), (2657.3 +/- 51.9), (1498.3 +/- 4.8), (4696.8 +/- 45.5), and (1200.5 +/- 42.2) pg/(10(6)cell.24 h). Intracellular bFGF expressions of SW1990, Capan-1, Aspc-1, MiaPaCa-2, Panc-1 and PCT-3 were (66.1 +/- 4.8), (206.8 +/- 99.5), (1532.0 +/- 54.6), (159.2 +/- 11.0), (1612.0 +/- 515.9) and (2781.2 +/- 479.0) pg/(10(6)cell.24 h). Extracellular bFGF expressions of SW1990, Capan-1, Aspc-1, MiaPaCa-2, Panc-1 and PCT-3 were (2.1 +/- 0.6), (10.3 +/- 1.5), (31.0 +/- 0.4), (4.3 +/- 1.2), (43.6 +/- 1.5) and (82.1 +/- 10.4) pg/(10(6)cell.24 h). Intracellular endostatin expressions of SW1990, Capan-1, Aspc-1, MiaPaCa-2, Panc-1 and PCT-3 were (0.2 +/- 0.0), (0.3 +/- 0.0), (4.7 +/- 0.1), (10.8 +/- 0.2), (31.9 +/- 11.7) and (5.4 +/- 0.1) ng/(10(6)cell.24 h). Extracellular endostatin expressions of SW1990, Capan-1, Aspc-1, MiaPaCa-2, Panc-1 and PCT-3 were (0.0 +/- 0.0), (1.6 +/- 0.0), (21.5 +/- 1.1), (40.8 +/- 0.4), (129.2 +/- 1.0) and (20.1 +/- 1.8) ng/(10(6)cell.24 h). Panc-1 enjoying stronger invasion and proliferation showed stronger expressions of VEGF, bFGF and endostatin. Intracellular expressions of bFGF was stronger than extracellular, extracellular expressions of VEGF and endostatin were stronger than intracellular.
Conclusion:
Expressions of angiogenic factors regulated by cancer cell played an important role in progression of pancreatic carcinoma.
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