Related Experiment Video
Updated: Jun 21, 2026

High-throughput Measurement of Plasma Membrane Resealing Efficiency in Mammalian Cells
Published on: January 7, 2019
Soluble CD146 is generated by ectodomain shedding of membrane CD146 in a calcium-induced, matrix
Eva-Maria Boneberg1, Harald Illges, Daniel F Legler
1Biotechnology Institute Thurgau, Unterseestrasse 47, Kreuzlingen 8280, Switzerland. eva.maria.boneberg@bitg.ch
Abstract:
CD146 is a cell adhesion molecule localized at the endothelial junction and is involved in the control of cell-cell cohesion. In this study, we showed that calcium influx in human microvascular lung endothelial cells results in the loss of surface CD146 and the release of soluble CD146. This calcium-induced CD146 shedding could be prevented with inhibitors of matrix metalloproteases indicating a central role of matrix metalloproteases in this process. We also investigated if CD146 shedding influences vascular permeability. Endothelial cell monolayers cultured on filter membranes showed an increased permeability for albumin when stimulated with ionomycin. This calcium-induced increase in permeability was inhibited when CD146 shedding was prevented by a matrix metalloprotease inhibitor. Our data indicate that surface CD146 plays a central role in the regulation of vascular permeability and demonstrate that CD146 and matrix metalloproteases are potential targets to modify endothelial barrier function.
Related Concept Videos
Role of Matrix Metalloproteases in Degradation of ECM
A...
Selectins
Structure of Cadherins
Receptor-mediated Endocytosis
Adherens Junctions
Adherens Junctions are Dynamic
The endothelial cells...
Intracellular Signaling Affects Focal Adhesions
Some...

