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Updated: Jun 21, 2026

NMR 15N Relaxation Experiments for the Investigation of Picosecond to Nanoseconds Structural Dynamics of Proteins
Published on: November 1, 2024
Using multiple quantum coherence to increase the 15N resolution in a three-dimensional TROSY HNCO experiment for
Kaifeng Hu1, Michaeleen Doucleff, G Marius Clore
1Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892-0520, USA.
Abstract:
We present a new version of the 3D TROSY HNCO pulse scheme, referred to as HR-TROSY HNCO, with comparable resolution in the (15)N dimension to a 2D (1)H-(15)N HSQC experiment. In the conventional 3D TROSY HNCO, the constant time period (1/2J(NC) approximately 32 ms) severely limits the maximum resolution in the (15)N dimension. In the HR-TROSY HNCO experiment presented here, both constant time periods (approximately 32 ms each) for coherence forward and backward transfer between (15)N and (13)C' are utilized to double the (15)N evolution time. This leads to a dramatic enhancement in peak separation along the (15)N dimension, making the HR-TROSY HNCO an ideal pulse scheme for accurate paramagnetic relaxation enhancement and residual dipolar coupling measurements.
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