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Related Experiment Video

Updated: Jun 21, 2026

Subcellular Fractionation for ERK Activation Upon Mitochondrial-derived Peptide Treatment

Subcellular Fractionation for ERK Activation Upon Mitochondrial-derived Peptide Treatment

Published on: September 25, 2017

Ras/MAPK signaling from endomembranes.

Nicole Fehrenbacher¹, Dafna Bar-Sagi, Mark Philips

¹Department of Cell Biology, NYU School of Medicine, New York, NY 10016, USA.

Molecular Oncology

|July 21, 2009

Summary

The Ras/MAPK pathway, crucial for cell signaling, occurs not only at the plasma membrane but also on intracellular membranes like endosomes. This spatial compartmentalization allows for more complex signal outputs.

Area of Science:

Cell Biology
Molecular Biology
Signal Transduction

Background:

The Ras/Mitogen-Activated Protein Kinase (MAPK) pathway is a fundamental signaling cascade in cellular processes.
Traditionally, Ras/MAPK signaling was localized exclusively to the plasma membrane.

Purpose of the Study:

To investigate the localization of Ras/MAPK signaling beyond the plasma membrane.
To explore the role of intracellular membranes in Ras/MAPK signal induction.

Main Methods:

Utilized Ras-green fluorescent protein (GFP) fusion proteins.
Employed genetically encoded fluorescent probes to monitor Ras activation.

Main Results:

Demonstrated Ras/MAPK signaling events occurring on intracellular membranes.

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Identified endosomes, Golgi apparatus, and endoplasmic reticulum as sites of Ras/MAPK signaling.

Revealed spatial compartmentalization of the Ras/MAPK pathway within the cell.

Conclusions:

The plasma membrane is not the sole platform for Ras/MAPK signal initiation.
Intracellular membranes contribute to the spatial compartmentalization of Ras/MAPK signaling.
This compartmentalization may enable increased complexity in cellular signal output.