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A HindIII polymorphism of fibronectin gene is associated with nephrolithiasis

Metin Onaran1, Akin Yilmaz, Ilker Sen

  • 1Department of Urology, School of Medicine, Gazi University, Ankara, Turkey. monaran@gazi.edu

Urology
|July 21, 2009
PubMed

Insights

The fibronectin gene (FN1) HindIII polymorphism is significantly associated with calcium oxalate nephrolithiasis, suggesting it may serve as a genetic risk marker for kidney stones.

Area of Science:

  • Genetics
  • Nephrology
  • Molecular Biology

Background:

  • Calcium oxalate nephrolithiasis is a common kidney stone disease.
  • Genetic factors play a role in the etiology of nephrolithiasis.
  • Fibronectin (FN1) gene polymorphisms are potential contributors to stone formation.

Purpose of the Study:

  • To investigate the association between fibronectin gene (FN1) polymorphisms and calcium oxalate nephrolithiasis.
  • To identify FN1 gene polymorphisms as potential genetic risk factors for kidney stone disease.

Main Methods:

  • Genomic DNA from 143 nephrolithiasis patients and 154 controls was analyzed.
  • Polymerase chain reaction-restriction fragments length polymorphism was used to screen FN1 gene polymorphisms (HaeIII b, MspI, HindIII).
  • Allele and genotype frequencies were compared between patient and control groups.

Main Results:

  • No significant differences in genotype distributions were observed for HaeIII b and MspI polymorphisms.
  • The FF genotype for the HindIII polymorphism showed a significant association with calcium oxalate nephrolithiasis (P = .00202 and P = .00203).

Conclusions:

  • The HindIII polymorphism of the FN1 gene is strongly associated with calcium oxalate stone disease.
  • FN1 gene variants, particularly the HindIII polymorphism, are potential genetic markers for nephrolithiasis risk.
  • Further research into FN1's role in stone disease etiology is warranted.
Abstract

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