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Lensless Fluorescent Microscopy on a Chip

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Published on: August 17, 2011

Improving FAIMS sensitivity using a planar geometry with slit interfaces.

Ridha Mabrouki¹, Ryan T Kelly, David C Prior

¹Biological Sciences Division, Pacific Northwest National Laboratory, Richland, Washington 99352, USA.

Journal of the American Society for Mass Spectrometry

|July 21, 2009

Summary

This summary is machine-generated.

A new planar (p-) FAIMS device with slit apertures significantly enhances ion transmission for gas-phase ion analysis. This improved Field Asymmetric Waveform Ion Mobility Spectrometry (FAIMS) coupling boosts signal in ESI/FAIMS/MS without compromising resolution.

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Area of Science:

Analytical Chemistry
Spectrometry
Ion Mobility

Background:

Field Asymmetric Waveform Ion Mobility Spectrometry (FAIMS) is increasingly used for gas-phase ion analysis.
FAIMS is often coupled with mass spectrometry (MS) or drift tube ion mobility spectrometry.
Commercial FAIMS/MS systems typically use curved geometries for MS interfacing, despite planar designs' advantages.

Purpose of the Study:

To introduce a novel planar FAIMS design with enhanced ion transmission.
To improve the coupling of FAIMS with electrospray ionization (ESI) sources.
To increase ion signal in ESI/FAIMS/MS analyses.

Main Methods:

Development of a new planar FAIMS device featuring slit-shaped entrance and exit apertures.
Integration of the planar FAIMS with multi-emitter ESI sources.
Evaluation of ion transmission and signal enhancement in ESI/FAIMS/MS experiments.

Main Results:

The new planar FAIMS design substantially increases ion transmission into and out of the analyzer.
The slit interface effectively couples p-FAIMS to ESI sources, supporting higher liquid flow rates (up to 50 microL/min).
Overall ion signal in ESI/FAIMS/MS analyses improved by over an order of magnitude, with no loss in FAIMS resolution.

Conclusions:

The novel planar FAIMS design offers significant advantages for gas-phase ion analysis.
Slit apertures enhance ion throughput and MS interfacing capabilities.
This advancement improves the sensitivity of ESI/FAIMS/MS techniques.