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Nuclear Export of mRNA

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...

Nuclear Export of mRNA

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...

Directing Proteins to the Rough Endoplasmic Reticulum

Directing Proteins to the Rough Endoplasmic Reticulum

The organelle-specific signaling sequences direct proteins synthesized in the cytosol to their final destination like ER, mitochondria, peroxisomes, etc. Some of the proteins directed to ER are then trafficked via vesicles to other organelles within the cell or the extracellular environment through the Golgi complex. For example, the rough ER synthesizes soluble proteins for transportation to the lysosomes or secretion out of the cell. It can also synthesize transmembrane proteins that can...

Regulated mRNA Transport

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...

Regulated mRNA Transport

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...

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Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
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Related Experiment Video

Updated: Jun 21, 2026

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Published on: January 16, 2017

Getting the message across: cytoplasmic ribonucleoprotein complexes.

J Bailey-Serres¹, Reed Sorenson, Piyada Juntawong

¹Center for Plant Cell Biology, Department of Botany and Plant Sciences, University of California, Riverside, CA 92521-0124, USA.

Trends in Plant Science

|July 21, 2009

Summary

This summary is machine-generated.

Messenger RNA-ribonucleoprotein (mRNP) complexes control gene expression after transcription. Quantitative mRNP analysis reveals insights into cytoplasmic regulation of mRNA activity, localization, and translation.

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Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

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Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Method for the Isolation and Identification of mRNAs, microRNAs and Protein Components of Ribonucleoprotein Complexes from Cell Extracts using RIP-Chip

Published on: September 29, 2012

Area of Science:

Molecular Biology
Gene Expression Regulation

Background:

Post-transcriptional control by mRNA-ribonucleoprotein (mRNP) complexes is crucial for gene expression.
mRNPs regulate mRNA localization, translation, sequestration, and turnover in the cytoplasm.

Purpose of the Study:

To review quantitative assessment of mRNAs in cytoplasmic mRNPs, including polyribosomes.
To explore genetic and biochemical studies of cytoplasmic mRNPs and their regulatory networks.

Main Methods:

Quantitative assessment of mRNAs within distinct cytoplasmic mRNPs.
Review of genetic and biochemical studies on mRNP composition and function.

Main Results:

Quantitative mRNP analysis offers new perspectives on post-transcriptional regulation.
Studies reveal an integrated network of RNA-binding proteins fine-tuning gene expression.

Conclusions:

Cytoplasmic mRNPs are key regulators of gene expression.
Understanding mRNP networks provides insights into fine-tuning gene expression.