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Tritrichomonas foetus: budding from multinucleated pseudocysts.

Antonio Pereira-Neves1, Marlene Benchimol

  • 1Laboratório de Ultraestrutura Celular, Universidade Santa Ursula, Rua Jornalista Orlando Dantas 59, Botafogo, CEP 22231-010, Rio de Janeiro, Brazil.

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Summary

Tritrichomonas foetus, a cattle parasite, forms multinucleated cells under stress. These cells then bud off new flagellated parasites when conditions improve, revealing a unique reproductive strategy.

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Area of Science:

  • Veterinary Parasitology
  • Cell Biology
  • Protozoology

Background:

  • Tritrichomonas foetus causes trichomoniasis, a significant sexually transmitted disease in cattle.
  • The parasite typically exists as a flagellated trophozoite but can form pseudocysts under stress.

Purpose of the Study:

  • To investigate the unique pseudocyst mitosis in Tritrichomonas foetus.
  • To understand polymastigont formation and the budding process of new trophozoites from multinucleated cells.

Main Methods:

  • Video microscopy was employed to observe cellular processes.
  • Complementary techniques included immunofluorescence and transmission electron microscopy.

Main Results:

  • Under stress, T. foetus trophozoites form pseudocysts, leading to multinucleated cells (approx. 62%) with duplicated mastigont structures.
  • Nuclear division occurs without cytoplasmic division during pseudocyst mitosis.
  • Favorable conditions trigger the budding of new flagellated trophozoites from these multinucleated cells.

Conclusions:

  • The study elucidates the distinct cell division process and reproductive strategy of Tritrichomonas foetus under varying environmental conditions.
  • Understanding this mechanism is crucial for controlling trichomoniasis in cattle herds.