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Updated: Jun 21, 2026

TransFLP — A Method to Genetically Modify Vibrio cholerae Based on Natural Transformation and FLP-recombination
Published on: October 8, 2012
DNA binding proteins of the filamentous phages CTXphi and VGJphi of Vibrio cholerae
Alina Falero1, Andy Caballero, Beatriz Ferrán
1Department of Molecular Biology, National Center for Scientific Research, P.O. Box 6414, Ave. 25 y 158, Cubanacán, Playa, Ciudad de la Habana, Cuba. alina.falero@cnic.edu.cu
Abstract:
The native product of open reading frame 112 (orf112) and a recombinant variant of the RstB protein, encoded by Vibrio cholerae pathogen-specific bacteriophages VGJphi and CTXphi, respectively, were purified to more than 90% homogeneity. Orf112 protein was shown to specifically bind single-stranded genomic DNA of VGJphi; however, RstB protein unexpectedly bound double-stranded DNA in addition to the single-stranded genomic DNA. The DNA binding properties of these proteins may explain their requirement for the rolling circle replication of the respective phages and RstB's requirement for single-stranded-DNA chromosomal integration of CTXphi phage dependent on XerCD recombinases.
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