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Updated: Jun 21, 2026

An In Ovo Model for Testing Insulin-mimetic Compounds
Published on: April 23, 2018
Human insulin A-chain peptide analog(s) with in vitro biological activity
Guillaume Le Flem1, Julien Pecher, Valerie Le Flem-Bonhomme
1Laboratoire de Biochimie du Tissu Conjonctif, UPRES EA 3214, Faculté de Médecine, Caen Cedex, France. guillaumeleflem@hotmail.com
Abstract:
In a previous study, we showed that a synthetic human insulin 1-chain analog, named analog (3) was capable of mimicking in vitro effects of native insulin, including stimulation of cell proliferation, glucose uptake and glycogen synthesis. Here, we have synthesized three new analogs (6, 9, 12) of the human A-chain, bearing or not their N- or C-terminal residue, to determine the structural features which are responsible for their biological properties. In vitro experiments clearly demonstrated that the N-terminal part of the peptides is required for the biological activity of the molecules, suggesting its crucial role in the mechanism underlying the cellular effect. Our findings may help to better understand the mechanism of interaction between insulin and its receptor. In addition, the present data demonstrate that some mini-insulin derived from the A-chain can exert similar effects as native insulin. These small peptides may offer specific advantages over insulin in the definition of new strategies for diabetes treatment.
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