[Effect of androsterone on scavenger receptor class B type I in human vascular endothelial cells]
1Department of Biochemistry and Molecular Biology, Third Military Medical University, Chongqing 400038, China. fan620@gmail.com
Objective:
To investigate the effect of FXR on scavenger receptor class B type I (SR-BI) expression.
Methods:
Human vascular endothelium Eahy926 cells were treated with FXR agonist androsterone, and the specific target gene of FXR SHP mRNA was detected by RT-PCR. SR-BI mRNA and protein were determined using RT-PCR, real-time PCR and Western blotting.
Results:
The level of SHP mRNA in Eahy926 cells increased after androsterone treatment at different concentrations for 24 h, demonstrating FXR activation in the cells. RT-PCR, real-time PCR and Western blotting detected increased SR-BI expression at both mRNA and protein levels after FXR activation.
Conclusion:
FXR increases the expression of SR-BI in human vascular endothelium cells.
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