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Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing
Published on: October 16, 2018
Virtual terminator nucleotides for next-generation DNA sequencing
Jayson Bowers1, Judith Mitchell, Eric Beer
1Helicos BioSciences Corporation, Cambridge, Massachusetts, USA.
Abstract:
We synthesized reversible terminators with tethered inhibitors for next-generation sequencing. These were efficiently incorporated with high fidelity while preventing incorporation of additional nucleotides, and we used them to sequence canine bacterial artificial chromosomes in a single-molecule system that provided even coverage for over 99% of the region sequenced. This single-molecule approach generated high-quality sequence data without the need for target amplification and thus avoided concomitant biases.
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