Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Direct visualisation and measurement of lung microstructure reveal insights into extracellular matrix dysregulation in COPD.

Respiratory research·2026
Same author

Evaluation of the Ultima Genomics UG 100 sequencer for low-cost, high-sensitivity metagenomic pathogen detection from cerebrospinal fluid.

Microbiology spectrum·2026
Same author

FRONTIER-3: a randomised phase 2a study to investigate tozorakimab, an anti-interleukin-33 monoclonal antibody, in early-onset asthma.

ERJ open research·2026
Same author

Anifrolumab Treatment Leads to Rapid Reduction in Urinary Biomarkers of Intrarenal Inflammation in Lupus Nephritis: Results From the Phase 2 Randomized Trial.

Arthritis & rheumatology (Hoboken, N.J.)·2026
Same author

First-in-human study for londamocitinib (AZD4604), an inhaled selective JAK1 inhibitor.

The Journal of allergy and clinical immunology·2026
Same author

A novel SPECT-CT imaging platform for quantifying <i>in vivo</i> lung cytokine signals in COPD.

ERJ open research·2025
Same journal

Efficient evidence-based genome annotation with EviAnn.

Nature methods·2026
Same journal

ClairS: a deep-learning method for long-read tumor-normal pair somatic small variant calling.

Nature methods·2026
Same journal

RNAbpFlow: base pair-augmented SE(3) flow matching for conditional RNA 3D structure generation.

Nature methods·2026
Same journal

Spatio-DARLIN enables robust and efficient in situ lineage tracing in mice at single-cell resolution.

Nature methods·2026
Same journal

EasyGrid: a versatile platform for automated cryo-EM sample preparation and quality control.

Nature methods·2026
Same journal

Cloud-based microscope enables live neuroimaging for 24 h and beyond with worldwide access.

Nature methods·2026
See all related articles

Related Experiment Video

Updated: Jun 21, 2026

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing
10:18

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Published on: October 16, 2018

Virtual terminator nucleotides for next-generation DNA sequencing.

Jayson Bowers1, Judith Mitchell, Eric Beer

  • 1Helicos BioSciences Corporation, Cambridge, Massachusetts, USA.

Nature Methods
|July 22, 2009
PubMed
Summary
This summary is machine-generated.

Researchers developed novel reversible terminators for high-fidelity next-generation sequencing. This single-molecule method sequences DNA without amplification, yielding high-quality data and avoiding biases.

More Related Videos

Rapid Verification of Terminators Using the pGR-Blue Plasmid and Golden Gate Assembly
09:51

Rapid Verification of Terminators Using the pGR-Blue Plasmid and Golden Gate Assembly

Published on: April 25, 2016

Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

Related Experiment Videos

Last Updated: Jun 21, 2026

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing
10:18

Amplification of Near Full-length HIV-1 Proviruses for Next-Generation Sequencing

Published on: October 16, 2018

Rapid Verification of Terminators Using the pGR-Blue Plasmid and Golden Gate Assembly
09:51

Rapid Verification of Terminators Using the pGR-Blue Plasmid and Golden Gate Assembly

Published on: April 25, 2016

Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

Area of Science:

  • Molecular Biology
  • Genomics
  • Biotechnology

Background:

  • Next-generation sequencing (NGS) technologies are crucial for genomic research.
  • Current NGS methods often require target amplification, which can introduce biases.
  • Developing unbiased, high-fidelity sequencing methods is essential for accurate genetic analysis.

Purpose of the Study:

  • To synthesize and evaluate novel reversible terminators with tethered inhibitors for NGS.
  • To apply this new sequencing chemistry in a single-molecule system.
  • To assess the efficiency, fidelity, and bias of the new method.

Main Methods:

  • Synthesis of reversible terminators featuring tethered inhibitors.
  • Incorporation of these terminators into DNA strands during sequencing.
  • Application in a single-molecule sequencing system for canine bacterial artificial chromosomes.
  • Analysis of sequence data for coverage and fidelity.

Main Results:

  • Efficient and high-fidelity incorporation of the novel terminators.
  • Prevention of additional nucleotide incorporation, ensuring accurate sequencing.
  • Successful sequencing of canine bacterial artificial chromosomes in a single-molecule system.
  • Achieved even coverage for over 99% of the sequenced region.
  • Generated high-quality sequence data without amplification biases.

Conclusions:

  • The developed reversible terminators enable accurate, high-fidelity NGS.
  • Single-molecule sequencing with these terminators bypasses amplification biases.
  • This approach offers a robust method for generating high-quality genomic data.