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Published on: December 22, 2014
Generation of a conditional CREB Ser133Ala knockin mouse
Andrew D Wingate1, Kirsty J Martin, Chris Hunter
1MRC Protein Phosphorylation Unit, School of Life Sciences, University of Dundee, Dundee, United Kingdom.
Genesis (New York, N.Y. : 2000)
|July 22, 2009
Summary
Researchers created a new mouse model to study the CREB protein. This model allows for conditional knockin of a Ser133Ala mutation, enabling in vivo investigation of CREB regulation beyond phosphorylation.
Area of Science:
- Molecular Biology
- Genetics
- Neuroscience
Background:
- Cyclic AMP response element-binding protein (CREB) phosphorylation at Ser133 is a key regulator of its transcriptional activity.
- Emerging evidence suggests other regulatory mechanisms influence CREB function.
- Studying these alternative regulatory pathways in vivo is crucial for a comprehensive understanding of CREB signaling.
Purpose of the Study:
- To develop a conditional mouse model for investigating CREB function independent of Ser133 phosphorylation.
- To enable in vivo analysis of CREB activity in adult mice.
- To facilitate the study of alternative CREB regulatory inputs.
Main Methods:
- Generation of a mouse CREB locus with a Ser133 to alanine knockin mutation.
- Utilized a minigene strategy for conditional knockin using Cre recombinase.
- Administered Cre recombinase to T cells and neurons to induce mutation expression.
Main Results:
- Successfully generated a conditional knockin mouse model for CREB Ser133Ala mutation.
- Observed expression of the mutated CREB protein prior to Cre induction.
- Confirmed exclusive detection of the mutated CREB protein in T cells and neurons post-Cre expression.
Conclusions:
- The developed mouse model allows for conditional and in vivo study of CREB function.
- This model facilitates the investigation of CREB regulatory mechanisms independent of Ser133 phosphorylation.
- Future research can utilize this model to explore alternative CREB signaling pathways in various cell types.
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