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Related Experiment Videos

Recent advances in electron immunocytochemistry.

P Monaghan1

  • 1Institute of Cancer Research, Haddow Laboratories, Sutton, UK.

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
|January 1, 1991
PubMed
Summary
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Researchers are advancing ultrastructural antigen localization using electron microscopy. Low-temperature methods, particularly freeze substitution, show promise for fixation-sensitive antigens, utilizing colloidal gold markers.

Area of Science:

  • Electron microscopy
  • Immunohistochemistry
  • Cell biology

Background:

  • Ultrastructural antigen localization is crucial for understanding cellular processes.
  • Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) are key tools.
  • Colloidal gold-linked reagents are widely used electron-dense markers.

Purpose of the Study:

  • To review methods for ultrastructural antigen localization.
  • To highlight the utility of colloidal gold markers.
  • To discuss low-temperature techniques for sensitive antigens.

Main Methods:

  • Review of post-embedding techniques for electron microscopy.
  • Discussion of cryosectioning and low-temperature embedding (PLT) methods.
  • Exploration of freeze substitution in non-fixative solvents.

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Main Results:

  • Colloidal gold reagents are effective electron-dense markers for most antigens.
  • Low-temperature preparative stages are essential for post-embedding techniques.
  • Freeze substitution offers potential for fixation-sensitive antigens.

Conclusions:

  • No single technique is universally applicable for all antigens.
  • Low-temperature methods, especially freeze substitution, are vital for preserving delicate antigens.
  • Advancements in electron microscopy techniques continue to improve antigen localization.