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[Differentially expressed proteins between normal ovaries and ovarian cancer tissues screened by the protein chips]
Fei Chen1, Jia-Xin Yang, Ming Wu
1Department of Obstetrics and Gynecology, PUMC Hospital, CAMS and PUMC, Beijing 100730, China.
Objective:
To explore the differentially expressed proteins between normal ovaries and ovarian cancer tissues using the protein chips.
Methods:
Tissues of 11 epithelial ovarian cancer (EOC) and 11 matched normal ovaries were labeled with cy3 and cy5 fluorescent dyes and then were hybridized with 512 monoclonal protein antibody chips. The internally normalized ratio (INR) was calculated according to the intensity of fluorescence of each protein spots. The value of INR > 2.0 or < 0.7 was considered as the cut-value to filtrate the differentially expressed proteins between tissues of EOC and normal ovaries.
Results:
Thirty one differentially expressed proteins were found between tissues of EOC and normal ovaries, in which 17 up-regulated and 14 down-regulated proteins involved in the transcription, proliferation, signal conduction, and apoptosis of cells.
Conclusion:
Antibody chips can effectively screen the differentially expressed proteins between normal ovaries and ovarian cancer tissues.
