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Updated: Jun 21, 2026
Bile Duct Ligation in Mice: Induction of Inflammatory Liver Injury and Fibrosis by Obstructive Cholestasis
Published on: February 10, 2015
Surgically-induced bile duct injury is followed by early hepatic oxidative stress. A preliminary experimental study
Germana Lissidini1, Giuseppe Piccinni, Piero Portincasa
1Section of General Surgery A. De Blasi Department for the Application in Surgery of Innovative Technologies, University of Bari, Bari, Italy.
Background/Aims:
To study in the rat whether oxidative stress appears early in a model of short-term cholestasis induced by bile duct ligation.
Methodology:
Adult male rats underwent proximal bile duct ligation; controls underwent a sham operation. Animals were sacrificed at days 0, 1, 3, 7 and 10. Livers were removed for histological studies and biochemical analysis.
Results:
GSH and PSH levels declined earlier in mitochondria than cytosol. Data were analyzed for statistical significance by ANOVA analysis.
Conclusions:
This study represents an experimental model which helps in the interpretation of the damage induced by bile duct injury in humans emphasizing an early detection of bile duct injury after surgery to ensure appropriate treatment and optimal patient outcome.
