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Updated: Jun 21, 2026

Imaging Subcellular Structures in the Living Zebrafish Embryo

Imaging Subcellular Structures in the Living Zebrafish Embryo

Published on: April 2, 2016

Intravital imaging in zebrafish using quantum dots.

Sang Wook Son¹, Jae Hwan Kim, Su Hyun Kim

¹Department of Dermatology, College of Medicine, Korea University, Seoul, Korea.

Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [And] International Society for Digital Imaging of Skin (ISDIS) [And] International Society for Skin Imaging (ISSI)

|July 23, 2009

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Fluorescent quantum dots (QDs) enable detailed imaging of zebrafish vasculature and motor neurons. This nanotechnology reveals spatial relationships between these systems in living embryos, aiding developmental and skin research.

Area of Science:

Nanotechnology applications in developmental biology.
In vivo imaging techniques.

Background:

Fluorescent quantum dots (QDs) offer advanced imaging capabilities for cellular and molecular interactions.
Zebrafish embryos serve as a model for intravital imaging due to their suitability for studying vertebrate systems.

Purpose of the Study:

To explore the potential of QDs in skin research using zebrafish embryos.
To visualize the cardiovascular system in zebrafish using QDs as microangiography agents.
To investigate the relationship between the cardiovascular and nervous systems.

Main Methods:

Quantum Dot QD605 (green fluorescence) was employed as a microangiography contrast agent.
The olig2-Dsred transgenic zebrafish line (red fluorescent motor neurons) was used for dual-color imaging.

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Digital microscopy captured images of QD605-injected embryos.

Main Results:

Detailed images were obtained by combining QD605 green fluorescence with olig2-Dsred red fluorescence.
The spatial relationship between the vascular and nervous systems in zebrafish embryos was clearly visualized.

Conclusions:

Quantum dots are effective as bright microangiography agents in living embryos.
Zebrafish vascular and motor nervous systems exhibit similar overall trajectories but distinct segmented patterns.
QD imaging provides insights into the interplay between circulatory and neural networks.