Molecular and morphological characterization of Dothiorella casuarini sp. nov. and other Botryosphaeriaceae with diplodia-like conidia
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Updated: Jun 21, 2026

Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants
Published on: May 5, 2023
Juanita de Wet1, Bernard Slippers, Oliver Preisig
1Forestry and Agricultural Biotechnology Institute, Department of Microbiology and Plant Pathology, University of Pretoria, Pretoria 0002, South Africa. juanita.dewet@fabi.up.ac.za
Abstract:
After recent changes to the taxonomy of the Botryosphaeriaceae species with diplodia-like (= dark, ovoid, often pigmented) conidia are considered to belong to at least three genera including Diplodia, Lasiodiplodia and Dothiorella. In a recent molecular phylogenetic study it became apparent that two groups of isolates with diplodia-like conidia required taxonomic revision. One group of isolates originated from Cupressus sempervirens in Greece and Cyprus and had been identified as D. pinea f. sp. cupressi based on morphological characteristics. The other isolates originated from a Casuarina sp. in Australia and were superficially similar to those in the first group based on their morphologically similar diplodia-like conidia. The aim of this study was to resolve the taxonomy of these two groups of isolates by combining the information from the multiple gene genealogies with morphological characters. The results showed that the isolates from C. sempervirens in Greece and Cyprus represent D. cupressi. The isolates from Casuarina in Australia belong to the more distantly related genus Dothiorella and represent a distinct species that is described here as Do. casuarini sp. nov.
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