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Updated: Jun 21, 2026
Small-Scale Plasma Membrane Preparation for the Analysis of Candida albicans Cdr1-mGFPHis
Published on: June 13, 2021
[Function of ferric reductase FRP1 gene in Candida albicans]
Yong Liang1, Wen Zheng, Dongsheng Wei
1Key Laboratory of Molecular and Technology, Ministry of Education, Department of Microbiology, Nankai University, Tianjin 300071, China. hnsdly@yahoo.com.cn
Unlabelled:
The ability of iron acquisition in Candida albicans has an effect on its growth and pathogenesis. Ferric reductases are important components of high affinity iron acquisition system in C. albicans.
Objective:
The aim of this study was to elucidate the function of FRP1 (Ferric reductase protein).
Methods:
FRP1 gene expression was detected under low-iron and high-iron conditions by Northern blot. We used the method of PCR-directed gene disruption to construct frp 1 null mutant and then the phenotypes of frp1delta/delta mutant were characterized.
Results:
Low-iron condition induced FRP1 gene expression. frp1delta/delta mutant showed no growth on low-iron media compared to wild-type strains on solid plates.
Conclusion:
FRP1 protein is probably the main ferric reductase under low-iron condition.
