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[Study on HPLC fingerprint of Trachelospermum jasminoides]
Yiquan Liu1, Nengjiang Yu, Xuedong Yang
1School of Pharmaceutical and Technology, Tianjin University, Tianjin 300072, China.
Objective:
To establish HPLC fingerprint for identification and evaluation of the quality of Trachelospermum jasminoides.
Method:
The analysis was performed on a ZORBAX Eclipse XDB-C18 analytical column (4.6 mm x 150 mm, 5 microm) with H2O (A) and methanol (B) as mobile phases in gradient mode. The elutin conditions were 0-15 min, changed from 10% B to 30% B; 15-40 min, to 40% B; 40-60 min, to 60% B. The column temperature was set at 30 degrees C and the flow rate was 0.8 mL x min(-1) with the detection wavelength of 230 nm.
Result:
The HPLC chromatographic fingerprint of T. jasminoides, showing 19 characteristic peaks, was established from 14 lots of T. jasminoides. The similarity of every lot of T. jasminoides was calculated as good in general, the similarity of 10 lots was above 0.9 and the other 4 lots had low similarity.
Conclusion:
The chromatographic fingerprint of T. jasminoides with high characteristics and specificity can be used to control its quality.
