Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 21, 2026

Bacterial Detection & Identification Using Electrochemical Sensors

Bacterial Detection & Identification Using Electrochemical Sensors

Published on: April 23, 2013

Field-effect enzymatic amplifying detector with picomolar detection limit.

Yongki Choi, Siu-Tung Yau

Analytical Chemistry

|July 24, 2009

Summary

Voltage-controlled amplification significantly enhances enzymatic detector sensitivity, lowering the glucose detection limit to picomolar levels. This field-effect transistor-like operation allows precise manipulation of biocatalytic reactions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Layer-by-layer deposition of polyelectrolyte assemblies on crop seeds as a platform for active ingredient loading and controlled release.

RSC advances·2026

Same author

Mesenchymal Stem Cell Exosome-Mediated Delivery of Paclitaxel for Pancreatic Cancer Therapy.

Biomolecules·2026

Same author

Nanoparticles for Delivery of Encapsulated Drugs to Hypoxic Pancreatic Ductal Adenocarcinoma Cell Spheroids.

Molecular pharmaceutics·2026

Same author

A synthetic biomolecular condensate from plant proteins with controlled colloidal properties.

Journal of materials chemistry. B·2025

Same author

Mechanistic Underpinnings of Epigenetic Enzyme-Responsive Nanoparticles.

ACS applied bio materials·2025

Same author

Hypoxia-Responsive Polymersomes for Stemness Reduction in Patient-Derived Solid Tumor Spheroids.

ACS applied bio materials·2025

Area of Science:

Biotechnology
Electrochemistry
Enzyme Engineering

Background:

Enzymatic detectors offer high specificity but often suffer from limited sensitivity.
Achieving low detection limits for analytes like glucose is crucial for diagnostics and research.
Controlling biocatalytic processes with external stimuli remains an area of active investigation.

Discussion:

Demonstrated voltage-controlled amplification of output current in enzymatic detectors using immobilized glucose oxidase.
Applied voltage between gating and working electrodes significantly boosted biocatalytic current.
Achieved reversible control over current amplification, enabling tunable detector response.

Key Insights:

Lowered glucose detection limit from millimolar to picomolar levels.

More Related Videos

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

Published on: June 13, 2010

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Related Experiment Videos

Last Updated: Jun 21, 2026

Bacterial Detection & Identification Using Electrochemical Sensors

Bacterial Detection & Identification Using Electrochemical Sensors

Published on: April 23, 2013

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

Published on: June 13, 2010

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Enzyme biospecificity was maintained under applied electric fields.

Detector operates analogously to a field-effect transistor, manipulating redox enzymatic reactions.

Outlook:

Potential for developing highly sensitive biosensors for various analytes.
Exploration of electric field effects on other enzyme-catalyzed reactions.
Integration into microfluidic devices for advanced point-of-care diagnostics.