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Related Experiment Video

Updated: Jun 21, 2026

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
13:21

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Published on: May 4, 2012

Profiling human serum antibodies with a carbohydrate antigen microarray.

Oyindasola Oyelaran1, Lisa M McShane, Lori Dodd

  • 1Laboratory of Medicinal Chemistry, National Cancer Institute, Frederick, MD 21702, USA.

Journal of Proteome Research
|July 24, 2009
PubMed
Summary
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Single-cell glycome and transcriptome profiling enabled by a library of anti-glycan antibodies.

Cell chemical biology·2025

This study characterizes carbohydrate antigen arrays (glycan arrays) for human serum profiling, establishing assay reproducibility and evaluating biological variability. Antibody levels show stability over time and significant dependence on age and blood type.

Area of Science:

  • Biochemistry
  • Immunology
  • Analytical Chemistry

Background:

  • Carbohydrate antigen arrays (glycan arrays) enable high-throughput analysis of carbohydrate macromolecule interactions.
  • Understanding experimental and biological variability is crucial for accurate human serum profiling using glycan arrays.

Purpose of the Study:

  • To characterize a novel carbohydrate antigen array and assay for human serum profiling.
  • To evaluate experimental and biological variability, including interindividual and intraindividual temporal variations.
  • To assess the impact of demographic factors (age, race, sex, location, blood type) on antibody profiles against carbohydrate antigens.

Main Methods:

  • Optimization of assay conditions and development of a normalization strategy for glycan array analysis.

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Profiling Anti-Neu5Gc IgG in Human Sera with a Sialoglycan Microarray Assay
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Profiling Anti-Neu5Gc IgG in Human Sera with a Sialoglycan Microarray Assay

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Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals
08:49

Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals

Published on: February 14, 2019

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Last Updated: Jun 21, 2026

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
13:21

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Published on: May 4, 2012

Profiling Anti-Neu5Gc IgG in Human Sera with a Sialoglycan Microarray Assay
09:13

Profiling Anti-Neu5Gc IgG in Human Sera with a Sialoglycan Microarray Assay

Published on: July 13, 2017

Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals
08:49

Printed Glycan Array: A Sensitive Technique for the Analysis of the Repertoire of Circulating Anti-carbohydrate Antibodies in Small Animals

Published on: February 14, 2019

  • Profiling antibody responses in serum from 48 human subjects.
  • Conducting a longitudinal study with 7 serum donors to assess temporal antibody level variations.

    • Main Results:

    • Achieved high reproducibility with within-experiment CV of 10.8% and overall CV of 28.5%.
    • Identified significant associations between antibody levels and host factors, particularly age and blood type.
    • Demonstrated general stability of anti-carbohydrate antibody levels within individuals over 3-13 weeks.

    Conclusions:

    • The characterized glycan array assay provides reproducible and reliable human serum profiling.
    • Age and blood type are significant determinants of anti-carbohydrate antibody profiles.
    • Anti-carbohydrate antibody levels are generally stable within individuals over short periods, supporting their use in longitudinal studies.