Related Experiment Video

Updated: Jun 21, 2026

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput
10:29

Measuring Gene Expression in Bombarded Barley Aleurone Layers with Increased Throughput

Published on: March 30, 2018

Arabidopsis 14-3-3 lambda is a positive regulator of RPW8-mediated disease resistance

Xiaohua Yang1, Wenming Wang, Mark Coleman

  • 1Center for Biosystems Research, University of Maryland Biotechnology Institute, Rockville, MD 20850, USA.

The Plant Journal : for Cell and Molecular Biology
|July 24, 2009
PubMed

Insights

Researchers identified GF14lambda as a protein interacting with RPW8.2, enhancing plant resistance to powdery mildew. This interaction is crucial for both basal and RPW8-mediated immunity in Arabidopsis.

Area of Science:

  • Plant immunity
  • Molecular plant-pathogen interactions
  • Arabidopsis thaliana genetics

Background:

  • The RPW8 locus in Arabidopsis thaliana provides broad-spectrum resistance to powdery mildew fungal pathogens.
  • This resistance is mediated by the salicylic acid-dependent signaling pathway.
  • Understanding RPW8 protein regulation is key to elucidating plant defense mechanisms.

Purpose of the Study:

  • To identify regulatory proteins interacting with RPW8.2.
  • To investigate the role of identified interactors in plant resistance.
  • To characterize the functional significance of the RPW8.2 and GF14lambda interaction.

Main Methods:

  • Yeast two-hybrid screening using RPW8.2 as bait.
  • Pulldown assays to confirm protein interactions.
  • T-DNA insertion for gene knockdown and overexpression studies.

Main Results:

  • GF14lambda was identified as a specific interactor of RPW8.2, binding to its C-terminal domain.
  • Knocking down GF14lambda compromised both basal and RPW8-mediated resistance to powdery mildew.
  • Overexpression of GF14lambda induced cell death and enhanced salicylic acid-dependent resistance.

Conclusions:

  • GF14lambda positively regulates RPW8.2 function in plant defense.
  • GF14lambda plays a significant role in enhancing basal resistance against powdery mildew in Arabidopsis.
  • The RPW8.2-GF14lambda interaction is a critical component of plant immunity.

More Related Videos

Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem11:50

Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem

Published on: October 1, 2015

Lateral Root Inducible System in Arabidopsis and Maize09:23

Lateral Root Inducible System in Arabidopsis and Maize

Published on: January 14, 2016

Related Concept Videos

Cell Signaling in Plants01:25

Cell Signaling in Plants

Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
Riboswitches01:56

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...