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Updated: Jun 21, 2026
Whole Blood Assay with Dual Co-Stimulation for Antigen-Specific Analysis of Host Immunity to Fungal and Viral Pathogens
Published on: September 20, 2024
Optimization of the detection of microbes in blood from immunocompromised patients with haematological malignancies
S Skovbjerg1, C Welinder-Olsson, N Kondori
1Department of Clinical Bacteriology, Sahlgrenska University Hospital, Göteborg, Sweden. susann.skovbjerg@vgregion.se
Abstract:
The present study aimed to improve the rate of detection of blood-borne microbes by using PCRs with pan-bacterial and Candida specificity. Seventeen per cent of the blood samples (n=178) collected from 107 febrile patients with haematological malignancies were positive using standard culture (BacT/Alert system). Candida PCR was positive in 12 patients, only one of whom scored culture-positive. Bacterial PCR using fresh blood samples was often negative, but the detection rate increased when the blood was pre-incubated for 2 days. These data indicate that PCR assays might be a complement for the detection of blood-borne opportunists in immunocompromised haematology patients.
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