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[Typing of methicillin-resistant Staphylococcus aureus with a specific type of resistance]
D Jovanović1, B Tomanović, Z Lepsanović
1Vojnomedicinska akademija, Zavod za preventivnu medicinu.
Abstract:
Methycillin resistant Staphylococcus aureus (MRSA) is the important causative agent of intrahospital infections (IHI) which could have epidemic character. Typization of strains is necessary for control of these infections. Fifty eight strains of MRSA with specific resistance to antibacterial agents have been isolated from patients at different clinics of our hospital. The determination of resistotype, biotype and plasmid profiles of these and control strains of Staphylococcus aureus (SA) was aimed at confirmation whether identical strains were in questions. Biotypization showed no difference between the studied and control strains while determination of the plasmid profile proved the presence of an epidemic strain which caused infection in several clinics of our hospital.
Insights
Methicillin-resistant Staphylococcus aureus (MRSA) can cause hospital infections. Plasmid profiling identified a specific epidemic MRSA strain responsible for infections across multiple hospital clinics, aiding in infection control.
Area of Science:
- Microbiology
- Infectious Diseases
- Epidemiology
Context:
- Methicillin-resistant Staphylococcus aureus (MRSA) is a significant cause of hospital-acquired infections.
- Controlling the spread of MRSA within healthcare settings is crucial.
- Strain typing is essential for understanding and managing MRSA outbreaks.
Purpose:
- To investigate the genetic relatedness of MRSA strains isolated from hospital patients.
- To determine if a single epidemic strain was responsible for infections in multiple clinics.
- To compare resistotype, biotype, and plasmid profiles of clinical MRSA isolates.
Summary:
- Fifty-eight MRSA strains were isolated and characterized alongside control Staphylococcus aureus (SA) strains.
- Biotypization revealed no significant differences between the studied and control strains.
- Plasmid profiling confirmed the presence of a single, epidemic MRSA strain circulating in several hospital clinics.
Impact:
- Identified a specific epidemic MRSA strain, facilitating targeted infection control measures.
- Demonstrated the utility of plasmid profiling in tracking the source and spread of hospital-acquired infections.
- Provided valuable data for hospital infection control protocols and strategies to combat MRSA.