Related Experiment Video
Updated: Jun 21, 2026

Impact of Common Fruit Juices and Toothbrushing on the Discoloration of Primary Teeth
Published on: March 27, 2026
Color change of vital teeth exposed to bleaching performed with and without supplementary light
Joe C Ontiveros1, Rade D Paravina
1Department of Restorative Dentistry and Biomaterials, The University of Texas Dental Branch at Houston, Houston, TX 77030, USA. joe.c.ontiveros@uth.tmc.edu
Objectives:
The aim of this clinical study was to evaluate tooth color change after exposure to 25% hydrogen peroxide in-office tooth whitening system, with and without supplementary light exposure.
Methods:
Twenty subjects were treated with two separate 45-min exposures of bleach, with and without light using an opposing-arch design. Visual and instrumental color measurements were obtained from eighty teeth before bleaching and seven days after treatment using two different shade guides, Vitapan Classical (VC), Vita Bleachedguide 3D-Master (BG) and an intraoral spectrophotometer. Data were analyzed using ANOVA, paired t-test, and Wilcoxon signed rank tests at the 0.05 level of significance.
Results:
Instrumental method revealed significant difference in color between treatment with light (DeltaE(ab)(*)=6.0) and without light (DeltaE(ab)(*)=4.7) after seven days (p<0.05). No differences were visually detected between treatment with light and without light using the VC (p=0.56). However, a significant difference was recorded using the BG (p<0.01). Instrumental measurements of color change were in better accordance with visual findings using the BG guide (R(2)=0.60) rather than the VC (R(2)=0.20).
Conclusions:
Within the limitations of this study, the treatment with supplementary light showed significantly greater bleaching-dependent changes in color compared to treatment without light when assessed using instrumental methods. The same was determined for the visual method with Vita Bleachedguide 3D-Master. No significant difference in color change with respect to light exposure was detected for the Vitapan Classical.
Related Concept Videos
Changes in Skin Color: Clinical Perspectives
Albinism
Albinism is a genetic disorder that affects (completely or partially) the coloring of skin, hair, and eyes. The defect is primarily...
Assessment of the Mouth
Mouth Inspection
The inspection begins with visually examining the mouth for symmetry, color, and size.
Pigmentation
Melanin occurs in two primary forms: eumelanin that provides black and brown pigment and pheomelanin that provides red color. Dark-skinned individuals produce more melanin than those with pale...
