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Ascorbic acid accumulation in human skin fibroblasts.

J D Butler1, P Bergsten, R W Welch

  • 1Human Genetics Branch, National Institute of Child Health and Human Development, Bethesda, MD 20892.

The American Journal of Clinical Nutrition
|December 1, 1991
PubMed
Summary
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Human skin fibroblasts actively transport and accumulate ascorbic acid (vitamin C) via two temperature-sensitive mechanisms, even against a concentration gradient.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Nutritional Science

Background:

  • Ascorbic acid, a vital antioxidant, plays a crucial role in skin health.
  • Understanding its cellular uptake is key to its therapeutic applications.

Purpose of the Study:

  • To investigate the mechanisms of ascorbic acid transport and accumulation in human skin fibroblasts.
  • To quantify ascorbic acid uptake and identify kinetic parameters.

Main Methods:

  • High-performance liquid chromatography (HPLC) with coulometric electrochemical detection.
  • Cultured normal human skin fibroblasts.
  • Kinetic analysis of ascorbic acid uptake over time.

Main Results:

  • Confluent fibroblasts initially showed undetectable ascorbic acid levels.

Related Experiment Videos

  • Cells accumulated a 15-fold excess of ascorbic acid within 3.5 hours upon incubation.
  • Kinetic studies revealed two distinct transport mechanisms: high-affinity and low-affinity.
  • Both transport systems were temperature-dependent and capable of active transport against a concentration gradient.

    • Conclusions:

    • Human skin fibroblasts possess active, temperature-sensitive mechanisms for ascorbic acid uptake.
    • These findings elucidate the cellular handling of vitamin C in skin, relevant for dermatological research and therapies.