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The role of ascorbate in the biosynthesis of neuroendocrine peptides
1Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205.
The American Journal of Clinical Nutrition
|December 1, 1991
Abstract:
The biosynthesis of all alpha-amidated peptides requires the participation of peptidylglycine alpha-amidating monooxygenase (PAM), a bifunctional enzyme dependent on molecular oxygen, ascorbate, and copper. In the AtT-20 corticotrope cell line, reduced concentrations of PAM and ascorbate can make alpha-amidation the rate-limiting step in the production of bioactive product peptide. Production of various peptides thought to play autocrine and paracrine roles may be affected by ascorbate availability.