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Ascorbic acid in human neutrophils.

P Washko1, D Rotrosen, M Levine

  • 1Laboratory of Cell Biology and Genetics, National Institute of Diabetes, Digestive, and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892.

The American Journal of Clinical Nutrition
|December 1, 1991
PubMed
Summary
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Human neutrophils accumulate high concentrations of ascorbic acid (vitamin C) via two transport systems. Extracellular glucose concentration-dependently inhibits this vitamin C uptake, but the effect is reversible.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Human Physiology

Background:

  • Ascorbic acid (vitamin C) is an essential nutrient crucial for various cellular functions.
  • Neutrophils play a vital role in the immune system and require adequate vitamin C levels.
  • Understanding the mechanisms of ascorbic acid transport in neutrophils is important for cellular health.

Purpose of the Study:

  • To investigate the uptake and distribution of ascorbic acid in human neutrophils.
  • To determine the effect of extracellular glucose on ascorbic acid transport in these cells.

Main Methods:

  • Isolation of human neutrophils.
  • Measurement of intracellular ascorbic acid concentrations.
  • Analysis of ascorbic acid transport kinetics.

Related Experiment Videos

  • Assessment of glucose inhibition on ascorbic acid uptake.

    • Main Results:

    • Human neutrophils contain high intracellular concentrations of reduced ascorbic acid (1.0-1.4 mmol/L).
    • Ascorbic acid accumulation is mediated by high- (Km 2-5 µmol/L) and low-affinity (Km 6-7 mmol/L) transport systems.
    • Extracellular glucose significantly inhibits ascorbic acid uptake and accumulation in a concentration-dependent manner.
    • Glucose-induced inhibition of ascorbic acid transport is fully reversible.

    Conclusions:

    • Human neutrophils possess efficient mechanisms for accumulating ascorbic acid, essential for their function.
    • Extracellular glucose can modulate ascorbic acid transport, potentially impacting neutrophil function.
    • The reversible nature of glucose inhibition suggests a dynamic regulatory interplay between glucose and vitamin C metabolism.