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Related Experiment Videos

Ascorbic and dehydroascorbic acid measurement in human serum and plasma.

S A Margolis1, R G Ziegler, K J Helzlsouer

  • 1National Institute of Standards & Technology, Gaithersburg, MD 20899.

The American Journal of Clinical Nutrition
|December 1, 1991
PubMed
Summary
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Ascorbic acid (vitamin C) stability in plasma was confirmed, and its measurement accuracy was established. The ascorbic acid to dehydroascorbic acid ratio in blood remains constant, suggesting physiological importance.

Area of Science:

  • Biochemistry
  • Clinical Chemistry

Background:

  • Ascorbic acid (vitamin C) is a vital nutrient with antioxidant properties.
  • Accurate measurement of ascorbic acid in biological samples is crucial for clinical assessment.
  • Understanding the stability and interconversion of ascorbic acid and its oxidized form, dehydroascorbic acid, is important.

Purpose of the Study:

  • To establish the accuracy and stability of ascorbic acid supplementation in plasma.
  • To evaluate methods for measuring ascorbic acid and identify sources of bias.
  • To assess the ratio of ascorbic acid to dehydroascorbic acid in clinical samples and its stability over time.

Main Methods:

  • Preparation and characterization of plasma supplemented with ascorbic acid.
  • Review of studies on the accuracy, precision, and methodological bias in ascorbic acid measurement.

Related Experiment Videos

  • Measurement of ascorbic acid and dehydroascorbic acid ratios in plasma from stored blood samples.

    • Main Results:

    • The accuracy of ascorbic acid supplementation in plasma was established.
    • Methodological biases in ascorbic acid measurement were summarized.
    • The ratio of ascorbic acid to dehydroascorbic acid in plasma remained constant in blood stored at 12°C for 6 hours.

    Conclusions:

    • Whole blood can maintain a stable ascorbic acid to dehydroascorbic acid ratio.
    • This stable ratio may hold physiological significance.
    • The findings support reliable clinical assessment of vitamin C status.