Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Fixation and Sectioning

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Academic bullying and diversity: challenges and solutions.

FEBS letters·2022

Same author

Proteoglycan 4 Reduces Neuroinflammation and Protects the Blood-Brain Barrier after Traumatic Brain Injury.

Journal of neurotrauma·2020

Same author

Recombinant Human Proteoglycan-4 Mediates Interleukin-6 Response in Both Human and Mouse Endothelial Cells Induced Into a Sepsis Phenotype.

Critical care explorations·2020

Same author

BDNF infusion into the MPN mag is sufficient to restore copulatory behavior in the castrated Syrian hamster.

Hormones and behavior·2018

Same author

TrkB is necessary for male copulatory behavior in the Syrian Hamster (Mesocricetus auratus).

Hormones and behavior·2017

Same author

Sexual dimorphic expression of TrkB, TrkB-T1, and BDNF in the medial preoptic area of the Syrian hamster.

Brain research·2017

Search research articles

Related Experiment Video

Updated: Jun 21, 2026

Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections

Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections

Published on: November 14, 2016

Temperature, peroxide concentration, and immunohistochemical staining method affects staining intensity,

Holly A Richendrfer¹, Jonathan A Wetzel, Jennifer M Swann

¹Department of Biological Sciences, Lehigh University, Bethlehem, PA, USA.

Applied Immunohistochemistry & Molecular Morphology : AIMM

|July 24, 2009

Summary

Robust neural tissue staining requires specific immunohistochemistry parameters. Storing tissue at very low temperatures and immersing it in staining solutions yields optimal results for both young and old samples.

More Related Videos

Immunohistochemistry Techniques to Analyze Cellular Proliferation and Neurogenesis in Rats Using the Thymidine Analog BrdU

Immunohistochemistry Techniques to Analyze Cellular Proliferation and Neurogenesis in Rats Using the Thymidine Analog BrdU

Published on: September 9, 2020

Deficient Pms2, ERCC1, Ku86, CcOI in Field Defects During Progression to Colon Cancer

Deficient Pms2, ERCC1, Ku86, CcOI in Field Defects During Progression to Colon Cancer

Published on: July 28, 2010

Related Experiment Videos

Last Updated: Jun 21, 2026

Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections

Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections

Published on: November 14, 2016

Immunohistochemistry Techniques to Analyze Cellular Proliferation and Neurogenesis in Rats Using the Thymidine Analog BrdU

Immunohistochemistry Techniques to Analyze Cellular Proliferation and Neurogenesis in Rats Using the Thymidine Analog BrdU

Published on: September 9, 2020

Deficient Pms2, ERCC1, Ku86, CcOI in Field Defects During Progression to Colon Cancer

Deficient Pms2, ERCC1, Ku86, CcOI in Field Defects During Progression to Colon Cancer

Published on: July 28, 2010

Area of Science:

Neuroscience
Immunohistochemistry

Background:

Immunostaining techniques for neural tissue vary, impacting results.
Neonatal neural tissue is particularly sensitive to processing methods.
Optimal tissue storage conditions can differ based on age.

Purpose of the Study:

To define optimal parameters for robust neural tissue staining.
To address challenges in processing both young and aged neural tissues.
To improve antigen labeling consistency in immunohistochemistry.

Main Methods:

Comparing free-floating versus slide-mounted tissue sections.
Investigating the effect of storage temperature on tissue integrity and staining.
Evaluating pretreatment with peroxide and immersion techniques during staining.

Main Results:

Slide-mounting is preferred for neonatal neural tissue to prevent damage.
Very low storage temperatures (-20°C and -80°C) enhance staining robustness.
Complete immersion in staining solutions improves antigen labeling.

Conclusions:

Specific storage temperatures and processing methods are crucial for successful neural tissue staining.
The developed parameters ensure reliable immunohistochemistry results across different age groups.
This study provides a standardized protocol for enhanced neural tissue antigen detection.