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Updated: Jan 19, 2026

Mouse Fetal Liver Culture System to Dissect Target Gene Functions at the Early and Late Stages of Terminal Erythropoiesis
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HIP1 exhibits an early recruitment and a late stage function in the maturation of coated pits

Irit Gottfried1, Marcelo Ehrlich, Uri Ashery

  • 1Department of Neurobiology, The George S. Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv, 69978 Tel Aviv, Israel.

Cellular and Molecular Life Sciences : CMLS
|July 24, 2009
PubMed

Insights

Huntingtin interacting protein 1 (HIP1) is crucial for clathrin-mediated endocytosis (CME) pit maturation and coated vesicle formation. Its membrane localization depends on clathrin, highlighting a key role in the CME pathway.

Area of Science:

  • Cell biology
  • Molecular and cell biology

Background:

  • Huntingtin interacting protein 1 (HIP1) is involved in clathrin-mediated endocytosis (CME).
  • Its specific function and timing within the CME pathway remain unclear.

Purpose of the Study:

  • To investigate the precise role of HIP1 in the early stages of CME.
  • To characterize HIP1 dynamics and its dependence on other CME components.

Main Methods:

  • Live-cell imaging techniques were utilized to observe HIP1 dynamics.
  • Transferrin receptor fused to pHluorin was employed to track endocytic events.
  • RNA interference (shRNA) was used to knock down clathrin expression.

Main Results:

  • HIP1 colocalizes with clathrin at the plasma membrane and exhibits similar dynamics.
  • HIP1 localizes to open endocytic pits but not to newly internalized vesicles.
  • Clathrin knockdown disrupts HIP1 membrane localization, unlike Sla2p.
  • A HIP1 fragment lacking specific domains inhibits transferrin internalization but remains associated with clathrin assemblies.

Conclusions:

  • HIP1 plays a significant role in the maturation of CME pits and the formation of coated vesicles.
  • HIP1's localization to the cell membrane is dependent on clathrin.

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