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Updated: Jun 21, 2026
Biosynthesis of a Flavonol from a Flavanone by Establishing a One-pot Bienzymatic Cascade
Published on: August 14, 2019
[Reparative isolation and purification of flavones from Pericarpium Citri Reticulatae by high-speed counter-current
Yinshi Sun1, Zhengbo Liu, Jianhu Wang
1College of Agronomy, Shandong Agricultural University, Taian 271018, China.
Abstract:
Three flavones were prepared, isolated and purified from Pericarpium Citri Reticulatae by high-speed counter-current chromatography (HSCCC). A two-phase solvent system composed of light petroleum-ethyl acetate-methanol-water (2:4:3:3, v/v/v/v) was used. Within 6 hours, 10.1 mg of heseridin, 49.8 mg of 5,6,7,8,4'-pentamethoxyflavone and 50.6 mg of 5-hydroxy-6,7,8,3',4'-pentamethoxyflavone with their purities over 97.0% were obtained from 4.0 g of the crude extract of Pericarpium Citri Reticulatae in one-step elution under the conditions of a flow rate of 1.70 mL/min, 800 r/min and the detection wavelength of 280 nm. The obtained fractions were analyzed by high performance liquid chromatography (HPLC), and identified by mass spectrometry (MS), 1H-nuclear magnetic resonance (NMR) and 13C-NMR. The results indicate that HSCCC is a powerful technique for the purification of flavones from Citrus reticulata Blanco.
