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[A fluorescent dye method based on changes in membrane potential for detecting PSP toxins in shellfish].

Li-juan Gao¹, Wei-dong Yang, Jie-sheng Liu

¹Department of Biotechnology, Jinan University, Guangzhou 510632, China. gaoli_138@163.com

Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu

|July 25, 2009

Summary

A new fluorimetric assay detects paralytic shellfish poisoning (PSP) toxins by measuring changes in cell membrane potential. This rapid, sensitive method accurately quantifies PSP toxins in shellfish, offering a reliable alternative to traditional bioassays.

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Area of Science:

Food Safety
Analytical Chemistry
Toxicology

Background:

Paralytic Shellfish Poisoning (PSP) poses a significant public health risk.
Accurate and sensitive detection of PSP toxins is crucial for seafood safety.
Existing detection methods, like the mouse bioassay, have limitations.

Purpose of the Study:

To develop a novel functional activity-based assay for screening PSP toxins.
To establish a rapid, specific, and reliable method for PSP toxin detection in shellfish.

Main Methods:

A fluorimetric assay utilizing T24 bladder cancer cells and the fluorescent dye bis-oxonol.
Monitoring changes in cell membrane potential following veratridine-induced depolarization.
Quantifying PSP toxin potency by measuring the inhibition of depolarization.

Main Results:

The assay demonstrated dose-dependent inhibition of depolarization by GTX2, 3 toxins (2-100 nmol x L(-1)).
A linear correlation was observed between toxin concentration and the percentage of depolarization inhibition.
PSP toxin levels in shellfish samples correlated well with the mouse bioassay but exhibited higher sensitivity.

Conclusions:

The developed fluorescent dye method provides a rapid, specific, and reliable approach for PSP toxin detection.
This functional assay offers a sensitive alternative to conventional methods for monitoring shellfish safety.
The method's reliance on membrane potential changes offers a unique mechanism for toxin screening.