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[MMP-9 expression profile in inflammatory cells of Mip-1alpha knockout mice and Mip-1alpha receptor knockout mice]
Yu Wu1, Naofumi Mukaida, Ting Liu
1Department of Hematology, West China Hospital, Sichuan University, Chengdu 610041, China.
Objective:
To explore the MMP-9 expression profile in peritoneal inflammatory macrophages and granulocytes in Mip-1alpha-, CCR1- and CCR5-deficient mice.
Methods:
In sodium thioglycolate-induced murine peritonitis models, peritoneal macrophages and granulocytes were harvested, identified and purified from WT mice and Mip-1alpha-, CCR1-, CCR5-deficient mice. The RT-PCR was applied to evaluate the expression of MMP-9 in macrophages and granulocytes of different group of mice.
Results:
The expressions of MMP-9 of macrophages in Mip-1alpha-, CCR1-, CCR5-deficient mice were significantly lower than that of WT mice (P<0.05); MMP-9 expression of granulocytes in Mip-1alpha-, CCR5-deficient mice were also significantly lower than that of WT mice (P<0.05), while the MMP-9 expression of granulocytes in CCR1-deficient mice was significant higher than that of WT mice.
Conclusion:
Deletion of Mip-1alpha and CCR5 could reduce the MMP-9 expression in both macrophages and granulocytes, while deletion of CCR1 could reduce MMP-9 expression in macrophages but increase MMP-9 expression in granulocytes.
Insights
Macrophage inflammatory protein 1-alpha (Mip-1alpha) and CCR5 deficiency reduce matrix metalloproteinase-9 (MMP-9) in immune cells. However, CCR1 deficiency decreases MMP-9 in macrophages but increases it in granulocytes.
Area of Science:
- Immunology
- Inflammation research
- Molecular biology
Background:
- Matrix metalloproteinase-9 (MMP-9) plays a role in inflammatory processes.
- Chemokine receptors CCR1 and CCR5, along with their ligand Mip-1alpha, are involved in immune cell recruitment and activation.
Purpose of the Study:
- To investigate the role of Mip-1alpha, CCR1, and CCR5 in regulating MMP-9 expression in peritoneal macrophages and granulocytes.
- To elucidate the specific contributions of these molecules to inflammatory responses.
Main Methods:
- Sodium thioglycolate-induced peritonitis model in mice.
- Isolation and purification of peritoneal macrophages and granulocytes from wild-type (WT) and Mip-1alpha-, CCR1-, CCR5-deficient mice.
- Quantitative reverse transcription polymerase chain reaction (RT-PCR) to assess MMP-9 gene expression.
Main Results:
- MMP-9 expression in macrophages was significantly lower in Mip-1alpha-, CCR1-, and CCR5-deficient mice compared to WT mice.
- MMP-9 expression in granulocytes was significantly lower in Mip-1alpha- and CCR5-deficient mice compared to WT mice.
- MMP-9 expression in granulocytes was significantly higher in CCR1-deficient mice compared to WT mice.
Conclusions:
- Mip-1alpha and CCR5 deficiency lead to reduced MMP-9 expression in both macrophages and granulocytes.
- CCR1 deficiency reduces MMP-9 expression in macrophages but increases it in granulocytes, indicating a complex regulatory role.
