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Related Experiment Video

Updated: Jun 21, 2026

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

Published on: October 8, 2021

Mixed protein carriers for modulating DNA release.

M Carmen Morán¹, Alberto A C C Pais, Amilcar Ramalho

¹Departamento de Quimica, Universidade de Coimbra, 3004-535 Coimbra, Portugal. mcarmen@qui.uc.pt

Langmuir : the ACS Journal of Surfaces and Colloids

|July 25, 2009

Summary

This summary is machine-generated.

Researchers created novel DNA gel particles using a mix of two proteins, improving DNA loading and enabling controlled release. This new method offers a versatile platform for DNA delivery applications.

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Last Updated: Jun 21, 2026

Preparation of Multifunctional Silk-Based Microcapsules Loaded with DNA Plasmids Encoding RNA Aptamers and Riboswitches

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Production of Double-stranded DNA Ministrings

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Published on: February 29, 2016

Area of Science:

Biomaterials Science
Polymer Chemistry
Biophysics

Background:

Associative phase separation of oppositely charged polyelectrolytes leads to coacervation, gelation, or precipitation.
This phenomenon is utilized for forming DNA gel particles via interfacial diffusion.

Purpose of the Study:

To report the formation of DNA gel particles by mixing double-stranded DNA with aqueous solutions of lysozyme and protamine sulfate.
To investigate the impact of the lysozyme/protamine ratio on DNA entrapment, particle morphology, swelling behavior, and DNA release kinetics.

Main Methods:

Formation of DNA gel particles by mixing DNA with dual cationic protein solutions (lysozyme and protamine sulfate).
Analysis of DNA loading efficiency, capacity, surface morphology, and swelling-deswelling properties.
Investigation of DNA release kinetics using zero-order, first-order, and Weibull models.
Fluorescence microscopy to assess DNA secondary structure preservation.

Main Results:

Mixed protein systems yielded higher DNA loading efficiency and capacity compared to single-protein systems.
Dual-stage, complex release kinetics were observed in mixed protein particles, dependent on the protein ratio.
DNA release profiles were accurately fitted using established kinetic models and the Weibull function.
Fluorescence microscopy indicated that DNA's secondary structure is preserved during particle formation.

Conclusions:

A novel platform for controlled DNA release from DNA gel particles formed by interfacial diffusion has been developed.
The lysozyme/protamine ratio critically influences particle properties and DNA release characteristics.
The findings suggest potential applications in advanced drug delivery and biomaterial design.