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A rapid screening method for bacteria degrading polycyclic aromatic hydrocarbons
1Department of Environmental Science and Engineering, State Key Joint Laboratory on Environmental Simulation and Pollution Control, Tsinghua University, Beijing, China.
Aims:
A rapid procedure was developed to screen for bacteria that are able to grow on polycyclic aromatic hydrocarbons (PAHs).
Methods And Results:
A drop of ethyl ether-dissolved PAH is spread on a sterilized cellulose acetate/nitrate filter lying on the top of a mineral salts agarose plate. After the evaporation of ethyl ether, a serially diluted sample is spread over the filter and incubated. Subsequently, the PAH degrading bacteria can be counted and isolated.
Conclusions:
This procedure is a simple method for screening bacterial isolates for the ability to grow with PAHs.
Significance And Impact Of The Study:
This technique is rapid to screen and/or count PAH-degrading bacteria and is also used to streak cultivation without disrupting the PAH layer on plate.
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