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Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
The Nucleolus02:55

The Nucleolus

The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
The Nucleolus02:55

The Nucleolus

The nucleolus is the most prominent substructure of the nucleus. When it was first discovered, it was considered to be an isolated organelle that forms fibrils and granules. In 1931, the relationship between the nucleolus and chromosomes was first described by Heitz. He observed that the appearance and size of nucleolus varies depending on the stage of the cell cycle. He also noticed constricted regions on different chromosomes clustered together at definite cell cycle stages. These regions,...
siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...

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Updated: Jun 21, 2026

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method
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MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as a Novel Detection and Quantification Method

Published on: October 7, 2025

MicroRNAs with a nucleolar location.

Joan C Ritland Politz1, Eric M Hogan, Thoru Pederson

  • 1Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01605, USA. jritland@fhcrc.org

RNA (New York, N.Y.)
|July 25, 2009
PubMed
Summary
This summary is machine-generated.

MicroRNAs (miRNAs) are found in the nucleolus, not just the cytoplasm. Some miRNAs concentrate in specific nucleolar sites, potentially involved in ribosome production or other nuclear functions.

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A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
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A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

Published on: August 21, 2019

Area of Science:

  • Molecular Biology
  • Cell Biology
  • RNA Biology

Background:

  • Noncoding RNAs, including microRNAs (miRNAs), are increasingly recognized for their nuclear functions.
  • Previous research identified the miRNA miR-206 concentrated in the nucleolus of rat myoblasts.

Purpose of the Study:

  • To investigate the presence and localization of various miRNAs within the nucleolus of rat myoblasts.
  • To characterize the form (precursor or mature) and specific localization of nucleolar miRNAs.
  • To explore potential origins and functions of nucleolar-concentrated miRNAs.

Main Methods:

  • Cell/nuclear fractionation followed by microarray analysis to detect miRNAs in the nucleolus.
  • RT-qPCR and in situ hybridization to further characterize specific miRNAs.
  • Homology searches against annotated rat snoRNAs.

Main Results:

  • Several miRNAs were detected in the nucleolus, with some showing significant concentration.
  • Nucleolar localization was specific, as other miRNAs were found predominantly in the nucleoplasm/cytoplasm.
  • Some nucleolar miRNAs exist as precursor forms, while others are mature species.
  • Nucleolar miRNAs are clustered in the granular component, with one showing homology to a snoRNA.

Conclusions:

  • The nucleolus is a site for specific miRNA localization, beyond their known cytoplasmic roles.
  • Nucleolar miRNAs may be processed from precursors or snoRNAs, or play roles in ribosome biogenesis or nonribosomal functions within the nucleolus.