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Creation of Murine Experimental Abdominal Aortic Aneurysms with Elastase
Published on: July 23, 2009
Creation of murine experimental abdominal aortic aneurysms with elastase
Junya Azuma1, Tomoko Asagami, Ronald Dalman
1Department of Cardiovascular Medicine, Stanford University School of Medicine. jazuma@stanford.edu
Journal of Visualized Experiments : Jove
|July 25, 2009
Summary
The elastase infusion model in mice effectively replicates abdominal aortic aneurysms (AAA). This method provides insights into AAA pathobiology and serves as a platform for testing potential therapies.
Area of Science:
- Cardiovascular Research
- Surgical Models
- Atherosclerosis Research
Background:
- Abdominal aortic aneurysm (AAA) is a significant vascular disease.
- The porcine pancreatic elastase infusion model is widely used to study AAA.
- Elastin disruption is a key feature in AAA pathogenesis.
Purpose of the Study:
- To demonstrate the elastase infusion procedure for creating an AAA model in mice.
- To highlight the utility of this model for AAA research.
- To provide a platform for testing potential AAA therapeutics.
Main Methods:
- Mice undergo anesthesia and sterile laparotomy.
- The infrarenal abdominal aorta is isolated and cannulated.
- The aorta is perfused with porcine pancreatic elastase to induce AAA.
Main Results:
- The procedure successfully creates an animal model of abdominal aortic aneurysm.
- This model allows for the study of AAA pathobiology.
- It serves as a platform for therapeutic agent testing.
Conclusions:
- The elastase infusion method is a valuable tool for AAA research in mice.
- This model facilitates the investigation of AAA pathogenesis.
- It supports the development and testing of novel AAA treatments.
