Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
The Mitotic Spindle02:27

The Mitotic Spindle

The mitotic spindle—or spindle apparatus—is a eukaryotic, cytoskeletal structure made up of long protein fibers called microtubules. Formed during cell division, the spindle separates sister chromatids and moves them to opposite ends of a parental cell, where the now individual chromosomes are distributed to two daughter cell nuclei.
The bipolar configuration of the mitotic spindle facilitates chromosomal segregation, preparing the cell for division. One mechanism that ensures bipolar mitotic...
The Mitotic Spindle02:27

The Mitotic Spindle

The mitotic spindle—or spindle apparatus—is a eukaryotic, cytoskeletal structure made up of long protein fibers called microtubules. Formed during cell division, the spindle separates sister chromatids and moves them to opposite ends of a parental cell, where the now individual chromosomes are distributed to two daughter cell nuclei.
The bipolar configuration of the mitotic spindle facilitates chromosomal segregation, preparing the cell for division. One mechanism that ensures bipolar mitotic...
Spindle Assembly02:50

Spindle Assembly

Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a microtubule array...
Spindle Assembly02:50

Spindle Assembly

Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a microtubule array...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

In vivo gene editing of human hematopoietic stem and progenitor cells using envelope-engineered virus-like particles.

Nature biotechnology·2025
Same author

In vivo chimeric antigen receptor (CAR)-T cell therapy.

Nature reviews. Drug discovery·2025
Same author

lncreased risk of slippage upon disengagement of the mitotic checkpoint.

PLoS computational biology·2025
Same author

Enhancing yeast cell tracking with a time-symmetric deep learning approach.

NPJ systems biology and applications·2025
Same author

Evolutionary adaptation to hyperstable microtubules selectively targets tubulins and is empowered by the spindle assembly checkpoint.

Cell reports·2025
Same author

Cell-targeted gene modification by delivery of CRISPR-Cas9 ribonucleoprotein complexes in pseudotyped lentivirus-derived nanoparticles.

Molecular therapy. Nucleic acids·2024

Related Experiment Video

Updated: Jun 21, 2026

Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes
10:09

Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes

Published on: September 13, 2022

A quantitative systems view of the spindle assembly checkpoint.

Andrea Ciliberto1, Jagesh V Shah

  • 1IFOM-Firc Institute of Molecular Oncology, Milan, Italy. andrea.ciliberto@ifom-ieo-campus.it

The EMBO Journal
|July 25, 2009
PubMed
Summary

The spindle assembly checkpoint ensures accurate chromosome segregation. This study proposes a systems view to understand how checkpoint proteins regulate this crucial cell division pathway.

More Related Videos

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
08:13

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

Live Cell Imaging of Chromosome Segregation During Mitosis
06:39

Live Cell Imaging of Chromosome Segregation During Mitosis

Published on: March 14, 2018

Related Experiment Videos

Last Updated: Jun 21, 2026

Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes
10:09

Evaluation of the Spindle Assembly Checkpoint Integrity in Mouse Oocytes

Published on: September 13, 2022

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
08:13

Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast

Published on: September 26, 2025

Live Cell Imaging of Chromosome Segregation During Mitosis
06:39

Live Cell Imaging of Chromosome Segregation During Mitosis

Published on: March 14, 2018

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Systems Biology

Background:

  • The spindle assembly checkpoint (SAC) is essential for accurate chromosome segregation during cell division.
  • It prevents premature anaphase until all chromosomes are properly attached to the mitotic spindle.
  • Despite identifying numerous SAC proteins, the precise communication mechanisms remain unclear.

Purpose of the Study:

  • To propose a systems-level perspective on the spindle assembly checkpoint.
  • To identify key regulators governing the dynamics of SAC signaling.
  • To bridge molecular function with pathway dynamics through interdisciplinary approaches.

Main Methods:

  • Review of existing cellular measurements and computational modeling studies.
  • Analysis of protein interactions and signaling dynamics.
  • Systems biology approach to understand pathway regulation.

Main Results:

  • Identified key regulators of SAC pathway dynamics.
  • Connected molecular functions of checkpoint proteins to signaling behaviors.
  • Highlighted the importance of quantitative approaches in understanding SAC.

Conclusions:

  • A systems view is crucial for deciphering SAC regulation.
  • Interdisciplinary studies combining experimental and computational methods are needed.
  • Further research is required to fully elucidate the quantitative underpinnings of this cellular control pathway.