Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fertilization01:38

Fertilization

93.9K
During fertilization, an egg and sperm cell fuse to create a new diploid structure. In humans, the process occurs once the egg has been released from the ovary, and travels into the fallopian tubes. The process requires several key steps: 1) sperm present in the genital tract must locate the egg; 2) once there, sperm need to release enzymes to help them burrow through the protective zona pellucida of the egg; and 3) the membranes of a single sperm cell and egg must fuse, with the sperm...
93.9K
Conservation of Protein Domains Over Different Proteins02:26

Conservation of Protein Domains Over Different Proteins

15.0K
Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...
15.0K
Protein-protein Interfaces02:04

Protein-protein Interfaces

15.0K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
15.0K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

3.1K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
3.1K
Protein-Protein Interfaces02:04

Protein-Protein Interfaces

4.6K
No description available
4.6K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

2.2K
No description available
2.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pacific bluefin tuna (Thunnus orientalis) bycatch in the Atlantic Ocean.

Journal of fish biology·2026
Same author

Comparative functional and evolutionary analysis of essential germline stem cell genes across the genus Drosophila and two outgroup species.

G3 (Bethesda, Md.)·2025
Same author

Microsatellite DNA markers for paternity identification in the hermaphroditic barnacle Chthamalus challengeri Hoek.

Molecular biology reports·2025
Same author

Comparative functional and evolutionary analysis of essential germline stem cell genes across the genus Drosophila and two outgroup species.

bioRxiv : the preprint server for biology·2025
Same author

Comparative Analysis of Drosophila Bam and Bgcn Sequences and Predicted Protein Structural Evolution.

Journal of molecular evolution·2025
Same author

Comparative Analysis of <i>Drosophila</i> Bam and Bgcn Sequences and Predicted Protein Structural Evolution.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: Apr 2, 2026

Evaluation of Fertilization State by Tracing Sperm Nuclear Morphology in Arabidopsis Double Fertilization
05:21

Evaluation of Fertilization State by Tracing Sperm Nuclear Morphology in Arabidopsis Double Fertilization

Published on: August 29, 2019

9.1K

Coevolution of interacting fertilization proteins.

Nathaniel L Clark1, Joe Gasper, Masashi Sekino

  • 1Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York, United States of America.

Plos Genetics
|July 25, 2009
PubMed
Summary

Abalone sperm and egg proteins show genetic associations and correlated evolution, supporting sexual selection theory. These findings reveal how coevolution drives reproductive barriers and speciation.

More Related Videos

Pulldown Assay Coupled with Co-Expression in Bacteria Cells as a Time-Efficient Tool for Testing Challenging Protein-Protein Interactions
07:03

Pulldown Assay Coupled with Co-Expression in Bacteria Cells as a Time-Efficient Tool for Testing Challenging Protein-Protein Interactions

Published on: December 23, 2022

3.8K
In Vivo Visualization of Calcium Transients during Fertilization and Early Development in C. elegans
05:11

In Vivo Visualization of Calcium Transients during Fertilization and Early Development in C. elegans

Published on: July 12, 2024

1.1K

Related Experiment Videos

Last Updated: Apr 2, 2026

Evaluation of Fertilization State by Tracing Sperm Nuclear Morphology in Arabidopsis Double Fertilization
05:21

Evaluation of Fertilization State by Tracing Sperm Nuclear Morphology in Arabidopsis Double Fertilization

Published on: August 29, 2019

9.1K
Pulldown Assay Coupled with Co-Expression in Bacteria Cells as a Time-Efficient Tool for Testing Challenging Protein-Protein Interactions
07:03

Pulldown Assay Coupled with Co-Expression in Bacteria Cells as a Time-Efficient Tool for Testing Challenging Protein-Protein Interactions

Published on: December 23, 2022

3.8K
In Vivo Visualization of Calcium Transients during Fertilization and Early Development in C. elegans
05:11

In Vivo Visualization of Calcium Transients during Fertilization and Early Development in C. elegans

Published on: July 12, 2024

1.1K

Area of Science:

  • Evolutionary biology
  • Genetics
  • Molecular biology

Background:

  • Reproductive proteins evolve rapidly due to positive selection.
  • Coevolution between interacting male and female proteins is a key driver of this rapid evolution.
  • Sexual selection theory predicts specific signatures of coevolution, such as allele association and correlated evolutionary rates.

Purpose of the Study:

  • To investigate female-male coevolution in abalone fertilization proteins, specifically sperm lysin and its interacting egg coat protein, VERL.
  • To test predictions of sexual selection theory regarding coevolutionary signatures.
  • To identify evidence for positive selection and its role in species-specific interactions.

Main Methods:

  • Resequencing of sperm lysin and VERL in abalone populations.
  • Analysis of intergenic linkage disequilibrium between lysin and VERL.
  • Development and application of a novel likelihood method to assess correlated evolutionary rates.
  • Polymorphism-based evidence for positive selection.

Main Results:

  • Found intergenic linkage disequilibrium between lysin and VERL, supporting allele association prediction.
  • Demonstrated correlated rates of evolution between lysin and VERL using a novel method.
  • Identified positive selection acting on specific structural regions of lysin and VERL.
  • Observed deep subdivision in VERL alleles, consistent with sexual conflict.

Conclusions:

  • Abalone fertilization proteins exhibit key signatures of coevolution, validating sexual selection theory.
  • Coevolution of reproductive proteins can lead to reproductive isolation and speciation.
  • The identified signatures can aid in predicting coevolving protein pairs.