Related Experiment Video
Updated: Jun 21, 2026
09:37
Navigating MARRVEL, a Web-Based Tool that Integrates Human Genomics and Model Organism Genetics Information
Published on: August 15, 2019
Target site selection by the mariner-like element, Mos1.
Gwénaelle Crénès1, Corinne Moundras, Marie-Véronique Demattei
1Université François Rabelais de Tours, GICC, Parc de Grandmont, 37200 Tours, France.
Genetica
|July 25, 2009
Summary
Mos1 transposon integration prefers TA dinucleotides within specific sequence motifs and AT-rich regions. Further research is needed to understand the physical properties influencing Mos1 target selection.
Area of Science:
- Molecular Biology
- Genetics
- Genomics
Background:
- The Mos1 transposon, a eukaryotic class-II transposable element, utilizes a "cut-and-paste" mechanism.
- While Mos1 excision is well-understood, its integration mechanism remains less investigated.
- Mos1 was previously believed to integrate randomly into TA dinucleotides.
Purpose of the Study:
- To synthesize current knowledge on Mos1 insertion sites.
- To define the characteristics of Mos1 insertion sequences in vitro and in vivo.
- To identify non-random TA dinucleotide targeting for transposon integration.
Main Methods:
- Statistical analysis of Mos1 insertion sites.
- In vitro studies of Mos1 integration.
- In vivo analysis of Mos1 integration hotspots in bacterial cat gene and C. elegans rDNA locus.
Main Results:
- In vitro, no target selection preference beyond TA dinucleotides was identified.
- In vivo, Mos1 showed a preference for TA dinucleotides within TATA or TA x TA motifs.
- A preference for AT-rich regions was also observed for in vivo Mos1 integration.
Conclusions:
- Mos1 integration exhibits sequence preferences in vivo, targeting specific motifs and AT-rich regions.
- Physical properties of sequences do not fully explain Mos1 integration preferences.
- Additional factors likely contribute to Mos1 target site selection.
Related Concept Videos
02:53Conservative Site-specific Recombination and Phase Variation
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
The recognition sites for Cre recombinase called LoxP...
01:22MOSFET: Enhancement Mode
Enhancement-mode MOSFETs are pivotal components in electronics, distinguished by their capacity to act as highly efficient switches. They are part of the larger family of metal-oxide Semiconductor Field-Effect Transistors (MOSFETs). They are available in two types: p-channel and n-channel, each tailored to specific polarity operations.
In their basic form, enhancement-mode MOSFETs are typically non-conductive when the gate-source voltage (Vgs) is zero. This default 'off' state means no current...
In their basic form, enhancement-mode MOSFETs are typically non-conductive when the gate-source voltage (Vgs) is zero. This default 'off' state means no current...
01:49Conserved Binding Sites
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally analyses the...
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally analyses the...