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Array-based proteomics: high-throughput expression and purification of IMAGE consortium cDNA clones.

J S Albala¹, I Humphery-Smith

¹Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, 7000 East Avenue L-452, Livermore, CA 94550, USA. albala1@llnl.gov

Current Opinion in Molecular Therapeutics

|July 25, 2009

Summary

Proteomics, the study of proteins, is crucial for understanding gene function following the Human Genome Project. Array-based methods enable high-throughput analysis of the human proteome for novel gene discovery.

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Area of Science:

Genomics and Proteomics
Molecular Biology
Biotechnology

Background:

The Human Genome Project is nearing completion, shifting focus to understanding gene function.
Protein analysis is essential for interpreting genomic data and understanding biological processes.
High-throughput genomic strategies have spurred the development of genome-scale protein science.

Purpose of the Study:

To outline the role of proteomics within the genomic sciences.
To emphasize array-based approaches in proteomics.
To introduce a high-throughput protein expression system for human proteome analysis.

Main Methods:

Development of a high-throughput protein expression system.
Application of array-based methodologies for proteomic analysis.

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Utilizing expressed sequence tag (EST) sequencing data.

Main Results:

A system for high-throughput analysis of the human proteome has been established.
Facilitation of improved functional and structural analysis of novel genes.
Integration of proteomics with genomic initiatives.

Conclusions:

Proteomics is the next frontier in understanding gene function post-genomic sequencing.
Array-based proteomics offers a powerful approach for genome-scale protein science.
The developed methodology supports the analysis of novel genes identified through genomic projects.