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[RAPD analysis for genetic diversity of Fagopyrum cymosum]
Chunping Zhang1, Ping He, Shijun Hu
1School of Life Sciences, Southwest University, Key Laboratory (Ministry of Education) of Eco-environments of Three Gorges Reservoir Region, Chongqing Key Laboratory of Plant Ecology , Chongqing, China.
Objective:
To study the genetic diversity of Fagopyrum cymosum.
Method:
The genetic diversity of 87 individuals from 10 populations was analyzed by random amplified polymorphic DNA (RAPD).
Result:
Twelve primers were selected to produce highly reproducible RAPD bands. Among 85 amplified bands, seventy-five showed polymorphism, the percentage of polymorphic bands reached to 88.24%. Nei's gene diversity index (H) was 0. 3103, Shannon information index (I) was 0.5632, Gst was 0.1924. The genetic similarity coefficient and the genetic distance were 0.6720-0.9678 and 0.0328-0.3975, respectively.
Conclusion:
F. cymosum shows high genetic diversity and the majority of genetic variation occurs in populations. By cluster analysis, the geographical distribution is very obvious. The RAPD marker can be used for the analysis of the genetic diversity and genetic variation of F. cymosum.
