Related Experiment Video
Updated: Jun 21, 2026

Minimal Erythema Dose (MED) Testing
Published on: May 28, 2013
Use of various models for in vitro percutaneous absorption studies of ultraviolet filters
Srei Pisei Huong1, Hot Bun, Jean-Dominique Fourneron
1Laboratoire de Pharmacie Galénique Industrielle et Cosmétologie, Université de la Méditerranée, Marseille, Cedex 05, France.
Background:
The percutaneous absorption test aims to estimate the passage of a substance across the skin. The absorption process can be described in three steps: (a) penetration of a substance into the skin layer, followed by (b) penetration from one layer into another (permeation) and finally (c) resorption into the vascular system. In vivo and in vitro models are available but owing to ethical reasons as well as the latter providing greater feasibility, in vitro models are preferred.
Aims:
This present study reviews the natural membranes (human skin and animal models: pig, rabbit, rat, hairless mouse, guinea-pig and mouse), artificial skin equivalents and synthetic membranes that are currently being used for in vitro percutaneous absorption studies of UV filters, in order to provide the researcher with a greater insight when selecting membrane models for given experimental conditions.
Related Concept Videos
Methods for Studying Drug Absorption: In vitro
The diffusion cell method uses a two-compartment cell, including a donor compartment with the drug solution, which simulates the environment where the drug is applied, and a receptor compartment with a buffer solution, which simulates the environment...
In Vitro Drug Dissolution: Compendial Testing Models II
Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview
Methods for Studying Drug Absorption: In situ
The Doluisio method involves perfusing a prepared segment of a rat's small intestine with a solution of radiolabeled drug and a non-absorbable marker. This helps to differentiate between absorbed and non-absorbed drug concentrations. The intestinal segment is connected at both ends using tubing and syringes,...
UV–Vis Spectrometers
In Vitro Drug Dissolution: Compendial Testing Models I

