Related Experiment Video
Updated: Jun 21, 2026
08:26
Analysis of N-glycans from Raphanus sativus Cultivars Using PNGase H+
Published on: June 25, 2018
Two novel oligosaccharides from Solanum nigrum.
Rong Chen1, Lin Feng, Hai-Dao Li
1Jiangsu Simcere Pharmaceutical R&D Co., Ltd, No. 699-18 Xuanwu Avenue, Xuanwu District, Nanjing 210042, PR China. blueoceanrr@yahoo.com.cn
Carbohydrate Research
|July 28, 2009
Summary
Researchers identified two new disaccharides from Solanum nigrum. These compounds, ethyl beta-D-thevetopyranosyl-(1-->4)-beta-D-oleandropyranoside and its alpha-D-isomer, were characterized using chemical and spectroscopic techniques.
Area of Science:
- Phytochemistry
- Organic Chemistry
- Natural Product Chemistry
Background:
- Solanum nigrum is a plant species with a history of traditional use.
- Phytochemical investigations aim to discover novel bioactive compounds.
- Disaccharides are complex carbohydrates with potential biological activities.
Purpose of the Study:
- To isolate and characterize novel disaccharides from Solanum nigrum.
- To elucidate the structures of the isolated compounds using advanced analytical methods.
Main Methods:
- Phytochemical analysis of Solanum nigrum extracts.
- Isolation of target compounds using chromatographic techniques.
- Structure elucidation via spectroscopic methods (e.g., NMR, Mass Spectrometry) and chemical derivatization.
Main Results:
- Two novel disaccharides were successfully isolated from Solanum nigrum.
- The structures were determined as ethyl beta-D-thevetopyranosyl-(1-->4)-beta-D-oleandropyranoside (1) and ethyl beta-D-thevetopyranosyl-(1-->4)-alpha-D-oleandropyranoside (2).
- Both compounds feature a beta-D-thevetopyranosyl unit linked to an oleandropyranoside moiety.
Conclusions:
- The study successfully identified and characterized two new disaccharide compounds from Solanum nigrum.
- The findings contribute to the understanding of the phytochemical profile of Solanum nigrum.
- Further research may explore the biological activities and potential applications of these novel disaccharides.
Related Concept Videos
01:24Oligosaccharide Assembly
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
Multiple sugar molecules that may or may...
01:26Biosynthesis of Polysaccharides
Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...
03:25Chemistry of Carbohydrates
Carbohydrates are an essential part of the diet in humans and animals. Grains, fruits, and vegetables are natural sources of carbohydrates that provide energy to the body, particularly through glucose, a simple sugar that is a component of starch and an ingredient in many staple foods. The stoichiometric formula (CH2O)n, where n is the number of carbons in the molecule represents carbohydrates. In other words, the ratio of carbon to hydrogen to oxygen is 1:2:1 in carbohydrate molecules. This...
01:25Protein Glycosylation
Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...
Glycosylation occurs in...
01:15Cellulose and Pectic Polysaccharides
Every plant cell has a cell wall that protects the cell, provides structural support, and gives the cell shape. Cellulose, the main structural component of the plant cell wall, makes up over 30% of plant matter. It is the most abundant organic compound on earth. Cellulose is an unbranched polysaccharide composed of linear chains of glucose molecules linked by β (1→4) glycosidic bonds.
As a cell matures, its cell wall specializes according to its type. For example, the parenchyma cells of...
As a cell matures, its cell wall specializes according to its type. For example, the parenchyma cells of...
01:27Glucose Transporters
Glucose transporters facilitate the transport of glucose across the cell membrane. In addition to glucose, some glucose transporters can also aid the movement of other hexoses such as fructose, mannose, and galactose.
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes: